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Microparticle traction force microscopy reveals subcellular force exertion patterns in immune cell–target interactions

Author

Listed:
  • Daan Vorselen

    (Stanford University
    University of Washington)

  • Yifan Wang

    (Stanford University)

  • Miguel M. Jesus

    (Memorial Sloan Kettering Cancer Center)

  • Pavak K. Shah

    (Sloan Kettering Institute)

  • Matthew J. Footer

    (Stanford University
    University of Washington)

  • Morgan Huse

    (Memorial Sloan Kettering Cancer Center)

  • Wei Cai

    (Stanford University)

  • Julie A. Theriot

    (Stanford University
    University of Washington)

Abstract

Force exertion is an integral part of cellular behavior. Traction force microscopy (TFM) has been instrumental for studying such forces, providing spatial force measurements at subcellular resolution. However, the applications of classical TFM are restricted by the typical planar geometry. Here, we develop a particle-based force sensing strategy for studying cellular interactions. We establish a straightforward batch approach for synthesizing uniform, deformable and tuneable hydrogel particles, which can also be easily derivatized. The 3D shape of such particles can be resolved with superresolution (

Suggested Citation

  • Daan Vorselen & Yifan Wang & Miguel M. Jesus & Pavak K. Shah & Matthew J. Footer & Morgan Huse & Wei Cai & Julie A. Theriot, 2020. "Microparticle traction force microscopy reveals subcellular force exertion patterns in immune cell–target interactions," Nature Communications, Nature, vol. 11(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-019-13804-z
    DOI: 10.1038/s41467-019-13804-z
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    Cited by:

    1. Alexander H. Settle & Benjamin Y. Winer & Miguel M. Jesus & Lauren Seeman & Zhaoquan Wang & Eric Chan & Yevgeniy Romin & Zhuoning Li & Matthew M. Miele & Ronald C. Hendrickson & Daan Vorselen & Justin, 2024. "β2 integrins impose a mechanical checkpoint on macrophage phagocytosis," Nature Communications, Nature, vol. 15(1), pages 1-20, December.
    2. Fabrizio A. Pennacchio & Alessandro Poli & Francesca Michela Pramotton & Stefania Lavore & Ilaria Rancati & Mario Cinquanta & Daan Vorselen & Elisabetta Prina & Orso Maria Romano & Aldo Ferrari & Matt, 2024. "N2FXm, a method for joint nuclear and cytoplasmic volume measurements, unravels the osmo-mechanical regulation of nuclear volume in mammalian cells," Nature Communications, Nature, vol. 15(1), pages 1-10, December.

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