IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v10y2019i1d10.1038_s41467-019-13468-9.html
   My bibliography  Save this article

Extracellular vesicles from symbiotic vaginal lactobacilli inhibit HIV-1 infection of human tissues

Author

Listed:
  • Rogers A. Ñahui Palomino

    (National Institutes of Health)

  • Christophe Vanpouille

    (National Institutes of Health)

  • Luca Laghi

    (University of Bologna)

  • Carola Parolin

    (University of Bologna)

  • Kamran Melikov

    (National Institutes of Health)

  • Peter Backlund

    (National Institutes of Health)

  • Beatrice Vitali

    (University of Bologna)

  • Leonid Margolis

    (National Institutes of Health)

Abstract

The vaginal microbiota, dominated by Lactobacillus spp., plays a key role in preventing HIV-1 transmission. Here, we investigate whether the anti-HIV effect of lactobacilli is mediated by extracellular vesicles (EVs) released by these bacteria. Human cervico-vaginal and tonsillar tissues ex vivo, and cell lines were infected with HIV-1 and treated with EVs released by lactobacilli isolated from vaginas of healthy women. EVs released by L. crispatus BC3 and L. gasseri BC12 protect tissues ex vivo and isolated cells from HIV-1 infection. This protection is associated with a decrease of viral attachment to target cells and viral entry due to diminished exposure of Env that mediates virus-cell interactions. Inhibition of HIV-1 infection is associated with the presence in EVs of several proteins and metabolites. Our findings demonstrate that the protective effect of Lactobacillus against HIV-1 is, in part, mediated by EVs released by these symbiotic bacteria. If confirmed in vivo, this finding may lead to new strategies to prevent male-to-female sexual HIV-1 transmission.

Suggested Citation

  • Rogers A. Ñahui Palomino & Christophe Vanpouille & Luca Laghi & Carola Parolin & Kamran Melikov & Peter Backlund & Beatrice Vitali & Leonid Margolis, 2019. "Extracellular vesicles from symbiotic vaginal lactobacilli inhibit HIV-1 infection of human tissues," Nature Communications, Nature, vol. 10(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-13468-9
    DOI: 10.1038/s41467-019-13468-9
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-019-13468-9
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-019-13468-9?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Nele De Langhe & Sofie Van Dorpe & Niké Guilbert & Amélie Vander Cruyssen & Quentin Roux & Sarah Deville & Sándor Dedeyne & Philippe Tummers & Hannelore Denys & Linos Vandekerckhove & Olivier De Wever, 2024. "Mapping bacterial extracellular vesicle research: insights, best practices and knowledge gaps," Nature Communications, Nature, vol. 15(1), pages 1-13, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-13468-9. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.