IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v10y2019i1d10.1038_s41467-019-13137-x.html
   My bibliography  Save this article

Independent control of the thermodynamic and kinetic properties of aptamer switches

Author

Listed:
  • Brandon D. Wilson

    (Stanford University)

  • Amani A. Hariri

    (Stanford University)

  • Ian A. P. Thompson

    (Stanford University)

  • Michael Eisenstein

    (Stanford University
    Stanford University)

  • H. Tom Soh

    (Stanford University
    Stanford University
    Chan Zuckerberg Biohub)

Abstract

Molecular switches that change their conformation upon target binding offer powerful capabilities for biotechnology and synthetic biology. Aptamers are useful as molecular switches because they offer excellent binding properties, undergo reversible folding, and can be engineered into many nanostructures. Unfortunately, the thermodynamic and kinetic properties of the aptamer switches developed to date are intrinsically coupled, such that high temporal resolution can only be achieved at the cost of lower sensitivity or high background. Here, we describe a design strategy that decouples and enables independent control over the thermodynamics and kinetics of aptamer switches. Starting from a single aptamer, we create an array of aptamer switches with effective dissociation constants ranging from 10 μM to 40 mM and binding kinetics ranging from 170 ms to 3 s. Our strategy is broadly applicable to other aptamers, enabling the development of switches suitable for a diverse range of biotechnology applications.

Suggested Citation

  • Brandon D. Wilson & Amani A. Hariri & Ian A. P. Thompson & Michael Eisenstein & H. Tom Soh, 2019. "Independent control of the thermodynamic and kinetic properties of aptamer switches," Nature Communications, Nature, vol. 10(1), pages 1-9, December.
  • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-13137-x
    DOI: 10.1038/s41467-019-13137-x
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-019-13137-x
    File Function: Abstract
    Download Restriction: no

    File URL: https://libkey.io/10.1038/s41467-019-13137-x?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Alex M. Yoshikawa & Alexandra E. Rangel & Liwei Zheng & Leighton Wan & Linus A. Hein & Amani A. Hariri & Michael Eisenstein & H. Tom Soh, 2023. "A massively parallel screening platform for converting aptamers into molecular switches," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    2. Nicolò Maganzini & Ian Thompson & Brandon Wilson & Hyongsok Tom Soh, 2022. "Pre-equilibrium biosensors as an approach towards rapid and continuous molecular measurements," Nature Communications, Nature, vol. 13(1), pages 1-15, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-13137-x. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.