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Structural transitions during the scaffolding-driven assembly of a viral capsid

Author

Listed:
  • Athanasios Ignatiou

    (Institute of Structural and Molecular Biology, Birkbeck College)

  • Sandrine Brasilès

    (Université Paris-Saclay)

  • Mehdi El Sadek Fadel

    (Université Paris-Saclay)

  • Jörg Bürger

    (Max-Planck-Institut für Molekulare Genetik, Microscopy and Cryo-Electron Microscopy Group
    Medizinische Physik und Biophysik, Charité – Universitätsmedizin Berlin)

  • Thorsten Mielke

    (Max-Planck-Institut für Molekulare Genetik, Microscopy and Cryo-Electron Microscopy Group)

  • Maya Topf

    (Institute of Structural and Molecular Biology, Birkbeck College)

  • Paulo Tavares

    (Université Paris-Saclay)

  • Elena V. Orlova

    (Institute of Structural and Molecular Biology, Birkbeck College)

Abstract

Assembly of tailed bacteriophages and herpesviruses starts with formation of procapsids (virion precursors without DNA). Scaffolding proteins (SP) drive assembly by chaperoning the major capsid protein (MCP) to build an icosahedral lattice. Here we report near-atomic resolution cryo-EM structures of the bacteriophage SPP1 procapsid, the intermediate expanded procapsid with partially released SPs, and the mature capsid with DNA. In the intermediate state, SPs are bound only to MCP pentons and to adjacent subunits from hexons. SP departure results in the expanded state associated with unfolding of the MCP N-terminus and straightening of E-loops. The newly formed extensive inter-capsomere bonding appears to compensate for release of SPs that clasp MCP capsomeres together. Subsequent DNA packaging instigates bending of MCP A domain loops outwards, closing the hexons central opening and creating the capsid auxiliary protein binding interface. These findings provide a molecular basis for the sequential structural rearrangements during viral capsid maturation.

Suggested Citation

  • Athanasios Ignatiou & Sandrine Brasilès & Mehdi El Sadek Fadel & Jörg Bürger & Thorsten Mielke & Maya Topf & Paulo Tavares & Elena V. Orlova, 2019. "Structural transitions during the scaffolding-driven assembly of a viral capsid," Nature Communications, Nature, vol. 10(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-12790-6
    DOI: 10.1038/s41467-019-12790-6
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    Cited by:

    1. Yashan Yang & Qianqian Shao & Mingcheng Guo & Lin Han & Xinyue Zhao & Aohan Wang & Xiangyun Li & Bo Wang & Ji-An Pan & Zhenguo Chen & Andrei Fokine & Lei Sun & Qianglin Fang, 2024. "Capsid structure of bacteriophage ΦKZ provides insights into assembly and stabilization of jumbo phages," Nature Communications, Nature, vol. 15(1), pages 1-12, December.

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