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Super-resolution imaging of fluorescent dipoles via polarized structured illumination microscopy

Author

Listed:
  • Karl Zhanghao

    (Peking University)

  • Xingye Chen

    (Tsinghua University)

  • Wenhui Liu

    (Tsinghua University)

  • Meiqi Li

    (Peking University)

  • Yiqiong Liu

    (Peking University)

  • Yiming Wang

    (Peking University)

  • Sha Luo

    (Chinese Academy of Sciences)

  • Xiao Wang

    (Peking University)

  • Chunyan Shan

    (Peking University)

  • Hao Xie

    (Tsinghua University)

  • Juntao Gao

    (Tsinghua University)

  • Xiaowei Chen

    (Peking University)

  • Dayong Jin

    (University of Technology Sydney)

  • Xiangdong Li

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Yan Zhang

    (Peking University)

  • Qionghai Dai

    (Tsinghua University)

  • Peng Xi

    (Peking University)

Abstract

Fluorescence polarization microscopy images both the intensity and orientation of fluorescent dipoles and plays a vital role in studying molecular structures and dynamics of bio-complexes. However, current techniques remain difficult to resolve the dipole assemblies on subcellular structures and their dynamics in living cells at super-resolution level. Here we report polarized structured illumination microscopy (pSIM), which achieves super-resolution imaging of dipoles by interpreting the dipoles in spatio-angular hyperspace. We demonstrate the application of pSIM on a series of biological filamentous systems, such as cytoskeleton networks and λ-DNA, and report the dynamics of short actin sliding across a myosin-coated surface. Further, pSIM reveals the side-by-side organization of the actin ring structures in the membrane-associated periodic skeleton of hippocampal neurons and images the dipole dynamics of green fluorescent protein-labeled microtubules in live U2OS cells. pSIM applies directly to a large variety of commercial and home-built SIM systems with various imaging modality.

Suggested Citation

  • Karl Zhanghao & Xingye Chen & Wenhui Liu & Meiqi Li & Yiqiong Liu & Yiming Wang & Sha Luo & Xiao Wang & Chunyan Shan & Hao Xie & Juntao Gao & Xiaowei Chen & Dayong Jin & Xiangdong Li & Yan Zhang & Qio, 2019. "Super-resolution imaging of fluorescent dipoles via polarized structured illumination microscopy," Nature Communications, Nature, vol. 10(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-12681-w
    DOI: 10.1038/s41467-019-12681-w
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    Cited by:

    1. Yanyi Song & Shuyi Jian & Junlin Teng & Pengli Zheng & Zhe Zhang, 2025. "Structural basis of human VANGL-PRICKLE interaction," Nature Communications, Nature, vol. 16(1), pages 1-13, December.

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