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Epi-illumination gradient light interference microscopy for imaging opaque structures

Author

Listed:
  • Mikhail E. Kandel

    (University of Illinois at Urbana-Champaign
    University of Illinois at Urbana-Champaign)

  • Chenfei Hu

    (University of Illinois at Urbana-Champaign
    University of Illinois at Urbana-Champaign)

  • Ghazal Naseri Kouzehgarani

    (University of Illinois at Urbana-Champaign
    University of Illinois at Urbana-Champaign)

  • Eunjung Min

    (Rowland Institute at Harvard University, Cambridge)

  • Kathryn Michele Sullivan

    (University of Illinois at Urbana-Champaign)

  • Hyunjoon Kong

    (University of Illinois at Urbana-Champaign
    University of Illinois at Urbana-Champaign
    University of Illinois at Urbana-Champaign
    University of Illinois at Urbana-)

  • Jennifer M. Li

    (Rowland Institute at Harvard University, Cambridge)

  • Drew N. Robson

    (Rowland Institute at Harvard University, Cambridge)

  • Martha U. Gillette

    (University of Illinois at Urbana-Champaign
    University of Illinois at Urbana-Champaign
    University of Illinois at Urbana-Champaign
    University of Illinois at Urbana-Champaign)

  • Catherine Best-Popescu

    (University of Illinois at Urbana-Champaign
    University of Illinois at Urbana-Champaign)

  • Gabriel Popescu

    (University of Illinois at Urbana-Champaign
    University of Illinois at Urbana-Champaign
    University of Illinois at Urbana-Champaign)

Abstract

Multiple scattering and absorption limit the depth at which biological tissues can be imaged with light. In thick unlabeled specimens, multiple scattering randomizes the phase of the field and absorption attenuates light that travels long optical paths. These obstacles limit the performance of transmission imaging. To mitigate these challenges, we developed an epi-illumination gradient light interference microscope (epi-GLIM) as a label-free phase imaging modality applicable to bulk or opaque samples. Epi-GLIM enables studying turbid structures that are hundreds of microns thick and otherwise opaque to transmitted light. We demonstrate this approach with a variety of man-made and biological samples that are incompatible with imaging in a transmission geometry: semiconductors wafers, specimens on opaque and birefringent substrates, cells in microplates, and bulk tissues. We demonstrate that the epi-GLIM data can be used to solve the inverse scattering problem and reconstruct the tomography of single cells and model organisms.

Suggested Citation

  • Mikhail E. Kandel & Chenfei Hu & Ghazal Naseri Kouzehgarani & Eunjung Min & Kathryn Michele Sullivan & Hyunjoon Kong & Jennifer M. Li & Drew N. Robson & Martha U. Gillette & Catherine Best-Popescu & G, 2019. "Epi-illumination gradient light interference microscopy for imaging opaque structures," Nature Communications, Nature, vol. 10(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-12634-3
    DOI: 10.1038/s41467-019-12634-3
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    Cited by:

    1. Xinwei Wang & Hao Wang & Jinlu Wang & Xingsi Liu & Huijie Hao & You Sin Tan & Yilei Zhang & He Zhang & Xiangyan Ding & Weisong Zhao & Yuhang Wang & Zhengang Lu & Jian Liu & Joel K. W. Yang & Jiubin Ta, 2023. "Single-shot isotropic differential interference contrast microscopy," Nature Communications, Nature, vol. 14(1), pages 1-9, December.
    2. Chenfei Hu & Shenghua He & Young Jae Lee & Yuchen He & Edward M. Kong & Hua Li & Mark A. Anastasio & Gabriel Popescu, 2022. "Live-dead assay on unlabeled cells using phase imaging with computational specificity," Nature Communications, Nature, vol. 13(1), pages 1-8, December.
    3. Felix Jünger & Dominic Ruh & Dominik Strobel & Rebecca Michiels & Dominik Huber & Annette Brandel & Josef Madl & Alina Gavrilov & Michael Mihlan & Caterina Cora Daller & Eva A. Rog-Zielinska & Winfrie, 2022. "100 Hz ROCS microscopy correlated with fluorescence reveals cellular dynamics on different spatiotemporal scales," Nature Communications, Nature, vol. 13(1), pages 1-17, December.

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