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Occurrence and repair of alkylating stress in the intracellular pathogen Brucella abortus

Author

Listed:
  • Katy Poncin

    (URBM, Narilis, University of Namur
    University of Oxford)

  • Agnès Roba

    (URBM, Narilis, University of Namur)

  • Ravikumar Jimmidi

    (University of Namur)

  • Georges Potemberg

    (URBM, Narilis, University of Namur)

  • Antonella Fioravanti

    (UMR 8576 CNRS, Université de Lille
    VIB,Vrije Universiteit Brussel)

  • Nayla Francis

    (URBM, Narilis, University of Namur)

  • Kévin Willemart

    (URBM, Narilis, University of Namur)

  • Nicolas Zeippen

    (URBM, Narilis, University of Namur)

  • Arnaud Machelart

    (URBM, Narilis, University of Namur
    Center for Infection and Immunity of Lille)

  • Emanuele G. Biondi

    (UMR 8576 CNRS, Université de Lille)

  • Eric Muraille

    (IMM, 31 Chemin Joseph Aiguier, 13009 Marseille, Aix-Marseille Université
    Université Libre de Bruxelles)

  • Stéphane P. Vincent

    (University of Oxford)

  • Xavier De Bolle

    (URBM, Narilis, University of Namur)

Abstract

It is assumed that intracellular pathogenic bacteria have to cope with DNA alkylating stress within host cells. Here we use single-cell reporter systems to show that the pathogen Brucella abortus does encounter alkylating stress during the first hours of macrophage infection. Genes encoding direct repair and base-excision repair pathways are required by B. abortus to face this stress in vitro and in a mouse infection model. Among these genes, ogt is found to be under the control of the conserved cell-cycle transcription factor GcrA. Our results highlight that the control of DNA repair in B. abortus displays distinct features that are not present in model organisms such as Escherichia coli.

Suggested Citation

  • Katy Poncin & Agnès Roba & Ravikumar Jimmidi & Georges Potemberg & Antonella Fioravanti & Nayla Francis & Kévin Willemart & Nicolas Zeippen & Arnaud Machelart & Emanuele G. Biondi & Eric Muraille & St, 2019. "Occurrence and repair of alkylating stress in the intracellular pathogen Brucella abortus," Nature Communications, Nature, vol. 10(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-12516-8
    DOI: 10.1038/s41467-019-12516-8
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    Cited by:

    1. Pierre Dupuy & Shreya Ghosh & Oyindamola Adefisayo & John Buglino & Stewart Shuman & Michael S. Glickman, 2022. "Distinctive roles of translesion polymerases DinB1 and DnaE2 in diversification of the mycobacterial genome through substitution and frameshift mutagenesis," Nature Communications, Nature, vol. 13(1), pages 1-13, December.

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