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Dietary fatty acids promote lipid droplet diversity through seipin enrichment in an ER subdomain

Author

Listed:
  • Zhe Cao

    (The Hong Kong University of Science and Technology)

  • Yan Hao

    (The Hong Kong University of Science and Technology)

  • Chun Wing Fung

    (The Hong Kong University of Science and Technology)

  • Yiu Yiu Lee

    (The Hong Kong University of Science and Technology)

  • Pengfei Wang

    (The Chinese University of Hong Kong)

  • Xuesong Li

    (The Hong Kong University of Science and Technology)

  • Kang Xie

    (Chinese Academy of Sciences)

  • Wen Jiun Lam

    (The Hong Kong University of Science and Technology)

  • Yifei Qiu

    (The Hong Kong University of Science and Technology)

  • Ben Zhong Tang

    (The Hong Kong University of Science and Technology)

  • Guanghou Shui

    (Chinese Academy of Sciences)

  • Pingsheng Liu

    (Chinese Academy of Sciences)

  • Jianan Qu

    (The Hong Kong University of Science and Technology)

  • Byung-Ho Kang

    (The Chinese University of Hong Kong)

  • Ho Yi Mak

    (The Hong Kong University of Science and Technology)

Abstract

Exogenous metabolites from microbial and dietary origins have profound effects on host metabolism. Here, we report that a sub-population of lipid droplets (LDs), which are conserved organelles for fat storage, is defined by metabolite-modulated targeting of the C. elegans seipin ortholog, SEIP-1. Loss of SEIP-1 function reduces the size of a subset of LDs while over-expression of SEIP-1 has the opposite effect. Ultrastructural analysis reveals SEIP-1 enrichment in an endoplasmic reticulum (ER) subdomain, which co-purifies with LDs. Analyses of C. elegans and bacterial genetic mutants indicate a requirement of polyunsaturated fatty acids (PUFAs) and microbial cyclopropane fatty acids (CFAs) for SEIP-1 enrichment, as confirmed by dietary supplementation experiments. In mammalian cells, heterologously expressed SEIP-1 engages nascent lipid droplets and promotes their subsequent expansion in a conserved manner. Our results suggest that microbial and polyunsaturated fatty acids serve unexpected roles in regulating cellular fat storage by promoting LD diversity.

Suggested Citation

  • Zhe Cao & Yan Hao & Chun Wing Fung & Yiu Yiu Lee & Pengfei Wang & Xuesong Li & Kang Xie & Wen Jiun Lam & Yifei Qiu & Ben Zhong Tang & Guanghou Shui & Pingsheng Liu & Jianan Qu & Byung-Ho Kang & Ho Yi , 2019. "Dietary fatty acids promote lipid droplet diversity through seipin enrichment in an ER subdomain," Nature Communications, Nature, vol. 10(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-10835-4
    DOI: 10.1038/s41467-019-10835-4
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    Cited by:

    1. Elodie Mailler & Carlos M. Guardia & Xiaofei Bai & Michal Jarnik & Chad D. Williamson & Yan Li & Nunziata Maio & Andy Golden & Juan S. Bonifacino, 2021. "The autophagy protein ATG9A enables lipid mobilization from lipid droplets," Nature Communications, Nature, vol. 12(1), pages 1-19, December.

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