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Three-dimensional view of ultrafast dynamics in photoexcited bacteriorhodopsin

Author

Listed:
  • Gabriela Nass Kovacs

    (Max-Planck-Institut für Medizinische Forschung)

  • Jacques-Philippe Colletier

    (Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale)

  • Marie Luise Grünbein

    (Max-Planck-Institut für Medizinische Forschung)

  • Yang Yang

    (Freie Universität Berlin, Department of Physics)

  • Till Stensitzki

    (Freie Universität Berlin, Department of Physics)

  • Alexander Batyuk

    (Linac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory)

  • Sergio Carbajo

    (Linac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory)

  • R. Bruce Doak

    (Max-Planck-Institut für Medizinische Forschung)

  • David Ehrenberg

    (Freie Universität Berlin, Department of Physics)

  • Lutz Foucar

    (Max-Planck-Institut für Medizinische Forschung)

  • Raphael Gasper

    (Max-Planck-Institut für Molekulare Physiologie)

  • Alexander Gorel

    (Max-Planck-Institut für Medizinische Forschung)

  • Mario Hilpert

    (Max-Planck-Institut für Medizinische Forschung)

  • Marco Kloos

    (Max-Planck-Institut für Medizinische Forschung)

  • Jason E. Koglin

    (Linac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory)

  • Jochen Reinstein

    (Max-Planck-Institut für Medizinische Forschung)

  • Christopher M. Roome

    (Max-Planck-Institut für Medizinische Forschung)

  • Ramona Schlesinger

    (Freie Universität Berlin, Department of Physics)

  • Matthew Seaberg

    (Linac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory)

  • Robert L. Shoeman

    (Max-Planck-Institut für Medizinische Forschung)

  • Miriam Stricker

    (Max-Planck-Institut für Medizinische Forschung)

  • Sébastien Boutet

    (Linac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory)

  • Stefan Haacke

    (Université de Strasbourg-CNRS, UMR 7504, IPCMS)

  • Joachim Heberle

    (Freie Universität Berlin, Department of Physics)

  • Karsten Heyne

    (Freie Universität Berlin, Department of Physics)

  • Tatiana Domratcheva

    (Max-Planck-Institut für Medizinische Forschung)

  • Thomas R. M. Barends

    (Max-Planck-Institut für Medizinische Forschung)

  • Ilme Schlichting

    (Max-Planck-Institut für Medizinische Forschung)

Abstract

Bacteriorhodopsin (bR) is a light-driven proton pump. The primary photochemical event upon light absorption is isomerization of the retinal chromophore. Here we used time-resolved crystallography at an X-ray free-electron laser to follow the structural changes in multiphoton-excited bR from 250 femtoseconds to 10 picoseconds. Quantum chemistry and ultrafast spectroscopy were used to identify a sequential two-photon absorption process, leading to excitation of a tryptophan residue flanking the retinal chromophore, as a first manifestation of multiphoton effects. We resolve distinct stages in the structural dynamics of the all-trans retinal in photoexcited bR to a highly twisted 13-cis conformation. Other active site sub-picosecond rearrangements include correlated vibrational motions of the electronically excited retinal chromophore, the surrounding amino acids and water molecules as well as their hydrogen bonding network. These results show that this extended photo-active network forms an electronically and vibrationally coupled system in bR, and most likely in all retinal proteins.

Suggested Citation

  • Gabriela Nass Kovacs & Jacques-Philippe Colletier & Marie Luise Grünbein & Yang Yang & Till Stensitzki & Alexander Batyuk & Sergio Carbajo & R. Bruce Doak & David Ehrenberg & Lutz Foucar & Raphael Gas, 2019. "Three-dimensional view of ultrafast dynamics in photoexcited bacteriorhodopsin," Nature Communications, Nature, vol. 10(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-10758-0
    DOI: 10.1038/s41467-019-10758-0
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    Citations

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    Cited by:

    1. Alma P. Perrino & Atsushi Miyagi & Simon Scheuring, 2021. "Single molecule kinetics of bacteriorhodopsin by HS-AFM," Nature Communications, Nature, vol. 12(1), pages 1-10, December.
    2. Volha U. Chukhutsina & James M. Baxter & Alisia Fadini & Rhodri M. Morgan & Matthew A. Pope & Karim Maghlaoui & Christian M. Orr & Armin Wagner & Jasper J. Thor, 2022. "Light activation of Orange Carotenoid Protein reveals bicycle-pedal single-bond isomerization," Nature Communications, Nature, vol. 13(1), pages 1-14, December.

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