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Bacteroidetes use thousands of enzyme combinations to break down glycans

Author

Listed:
  • Pascal Lapébie

    (Institut National Agronomique (INRA, USC 1408) and Aix-Marseille Université (AMU))

  • Vincent Lombard

    (Institut National Agronomique (INRA, USC 1408) and Aix-Marseille Université (AMU))

  • Elodie Drula

    (Institut National Agronomique (INRA, USC 1408) and Aix-Marseille Université (AMU))

  • Nicolas Terrapon

    (Institut National Agronomique (INRA, USC 1408) and Aix-Marseille Université (AMU))

  • Bernard Henrissat

    (Institut National Agronomique (INRA, USC 1408) and Aix-Marseille Université (AMU)
    King Abdulaziz University)

Abstract

Unlike proteins, glycan chains are not directly encoded by DNA, but by the specificity of the enzymes that assemble them. Theoretical calculations have proposed an astronomical number of possible isomers (> 1012 hexasaccharides) but the actual diversity of glycan structures in nature is not known. Bacteria of the Bacteroidetes phylum are considered primary degraders of polysaccharides and they are found in all ecosystems investigated. In Bacteroidetes genomes, carbohydrate-degrading enzymes (CAZymes) are arranged in gene clusters termed polysaccharide utilization loci (PULs). The depolymerization of a given complex glycan by Bacteroidetes PULs requires bespoke enzymes; conversely, the enzyme composition in PULs can provide information on the structure of the targeted glycans. Here we group the 13,537 PULs encoded by 964 Bacteroidetes genomes according to their CAZyme composition. We find that collectively Bacteroidetes have elaborated a few thousand enzyme combinations for glycan breakdown, suggesting a global estimate of diversity of glycan structures much smaller than the theoretical one.

Suggested Citation

  • Pascal Lapébie & Vincent Lombard & Elodie Drula & Nicolas Terrapon & Bernard Henrissat, 2019. "Bacteroidetes use thousands of enzyme combinations to break down glycans," Nature Communications, Nature, vol. 10(1), pages 1-7, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-10068-5
    DOI: 10.1038/s41467-019-10068-5
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    1. Felipe Papa Pellizoni & Aline Zazeri Leite & Nathália de Campos Rodrigues & Marcelo Jordão Ubaiz & Marina Ignácio Gonzaga & Nauyta Naomi Campos Takaoka & Vânia Sammartino Mariano & Wellington Pine Omo, 2021. "Detection of Dysbiosis and Increased Intestinal Permeability in Brazilian Patients with Relapsing–Remitting Multiple Sclerosis," IJERPH, MDPI, vol. 18(9), pages 1-17, April.
    2. Han, Yujie & Chang, Xiao & Xiang, Hai & Fang, Yi & Hao, Lizhuang & Gu, Yue & Han, Xinyu & Zhao, Bao & Zhao, Zijiao & Zhao, Chengzhen & Zhong, Rongzhen, 2023. "Exploring biomimetic potential of ruminant digestion strategies for lignocellulosic biomass utilization: A comprehensive review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 188(C).

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