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Conformational dynamics of the human serotonin transporter during substrate and drug binding

Author

Listed:
  • Ingvar R. Möller

    (University of Copenhagen
    University of Copenhagen)

  • Marika Slivacka

    (University of Copenhagen
    University of Copenhagen)

  • Anne Kathrine Nielsen

    (University of Copenhagen)

  • Søren G. F. Rasmussen

    (University of Copenhagen)

  • Ulrik Gether

    (University of Copenhagen)

  • Claus J. Loland

    (University of Copenhagen)

  • Kasper D. Rand

    (University of Copenhagen)

Abstract

The serotonin transporter (SERT), a member of the neurotransmitter:sodium symporter family, is responsible for termination of serotonergic signaling by re-uptake of serotonin (5-HT) into the presynaptic neuron. Its key role in synaptic transmission makes it a major drug target, e.g. for the treatment of depression, anxiety and post-traumatic stress. Here, we apply hydrogen-deuterium exchange mass spectrometry to probe the conformational dynamics of human SERT in the absence and presence of known substrates and targeted drugs. Our results reveal significant changes in dynamics in regions TM1, EL3, EL4, and TM12 upon binding co-transported ions (Na+/K+) and ligand-mediated changes in TM1, EL3 and EL4 upon binding 5-HT, the drugs S-citalopram, cocaine and ibogaine. Our results provide a comprehensive direct view of the conformational response of SERT upon binding both biologically relevant substrate/ions and ligands of pharmaceutical interest, thus advancing our understanding of the structure-function relationship in SERT.

Suggested Citation

  • Ingvar R. Möller & Marika Slivacka & Anne Kathrine Nielsen & Søren G. F. Rasmussen & Ulrik Gether & Claus J. Loland & Kasper D. Rand, 2019. "Conformational dynamics of the human serotonin transporter during substrate and drug binding," Nature Communications, Nature, vol. 10(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-09675-z
    DOI: 10.1038/s41467-019-09675-z
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    Cited by:

    1. Eva Hellsberg & Gerhard F Ecker & Anna Stary-Weinzinger & Lucy R Forrest, 2019. "A structural model of the human serotonin transporter in an outward-occluded state," PLOS ONE, Public Library of Science, vol. 14(6), pages 1-25, June.
    2. Andreas Nygaard & Linda G. Zachariassen & Kathrine S. Larsen & Anders S. Kristensen & Claus J. Loland, 2024. "Fluorescent non-canonical amino acid provides insight into the human serotonin transporter," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    3. Solveig G. Schmidt & Mette Galsgaard Malle & Anne Kathrine Nielsen & Søren S.-R. Bohr & Ciara F. Pugh & Jeppe C. Nielsen & Ida H. Poulsen & Kasper D. Rand & Nikos S. Hatzakis & Claus J. Loland, 2022. "The dopamine transporter antiports potassium to increase the uptake of dopamine," Nature Communications, Nature, vol. 13(1), pages 1-12, December.

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