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Structural basis for transcription initiation by bacterial ECF σ factors

Author

Listed:
  • Lingting Li

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Chengli Fang

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Ningning Zhuang

    (Chinese Academy of Sciences)

  • Tiantian Wang

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Yu Zhang

    (Chinese Academy of Sciences)

Abstract

Bacterial RNA polymerase employs extra-cytoplasmic function (ECF) σ factors to regulate context-specific gene expression programs. Despite being the most abundant and divergent σ factor class, the structural basis of ECF σ factor-mediated transcription initiation remains unknown. Here, we determine a crystal structure of Mycobacterium tuberculosis (Mtb) RNAP holoenzyme comprising an RNAP core enzyme and the ECF σ factor σH (σH-RNAP) at 2.7 Å, and solve another crystal structure of a transcription initiation complex of Mtb σH-RNAP (σH-RPo) comprising promoter DNA and an RNA primer at 2.8 Å. The two structures together reveal the interactions between σH and RNAP that are essential for σH-RNAP holoenzyme assembly as well as the interactions between σH-RNAP and promoter DNA responsible for stringent promoter recognition and for promoter unwinding. Our study establishes that ECF σ factors and primary σ factors employ distinct mechanisms for promoter recognition and for promoter unwinding.

Suggested Citation

  • Lingting Li & Chengli Fang & Ningning Zhuang & Tiantian Wang & Yu Zhang, 2019. "Structural basis for transcription initiation by bacterial ECF σ factors," Nature Communications, Nature, vol. 10(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-09096-y
    DOI: 10.1038/s41467-019-09096-y
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    Cited by:

    1. Alec Fraser & Maria L. Sokolova & Arina V. Drobysheva & Julia V. Gordeeva & Sergei Borukhov & John Jumper & Konstantin V. Severinov & Petr G. Leiman, 2022. "Structural basis of template strand deoxyuridine promoter recognition by a viral RNA polymerase," Nature Communications, Nature, vol. 13(1), pages 1-18, December.
    2. Linggang Yuan & Qingyang Liu & Liqiao Xu & Bing Wu & Yu Feng, 2024. "Structural basis of promoter recognition by Staphylococcus aureus RNA polymerase," Nature Communications, Nature, vol. 15(1), pages 1-9, December.
    3. Jie Li & Haonan Zhang & Dongyu Li & Ya-Jun Liu & Edward A. Bayer & Qiu Cui & Yingang Feng & Ping Zhu, 2023. "Structure of the transcription open complex of distinct σI factors," Nature Communications, Nature, vol. 14(1), pages 1-12, December.

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