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A threonyl-tRNA synthetase-mediated translation initiation machinery

Author

Listed:
  • Seung Jae Jeong

    (Seoul National University
    Seoul National University)

  • Shinhye Park

    (Korea Research Institute of Bioscience and Biotechnology (KRIBB))

  • Loi T. Nguyen

    (Korea Research Institute of Bioscience and Biotechnology (KRIBB)
    Chungnam National University)

  • Jungwon Hwang

    (Korea Research Institute of Bioscience and Biotechnology (KRIBB))

  • Eun-Young Lee

    (Korea Research Institute of Bioscience and Biotechnology (KRIBB))

  • Hoi-Khoanh Giong

    (Disease Target Structure Research Center, KRIBB
    Korea Institute of Science and Technology
    Korea University of Science and Technology)

  • Jeong-Soo Lee

    (Disease Target Structure Research Center, KRIBB
    Korea Institute of Science and Technology)

  • Ina Yoon

    (Seoul National University
    Seoul National University)

  • Ji-Hyun Lee

    (Seoul National University)

  • Jong Hyun Kim

    (Seoul National University)

  • Hoi Kyoung Kim

    (Seoul National University)

  • Doyeun Kim

    (Seoul National University)

  • Won Suk Yang

    (Seoul National University)

  • Seon-Young Kim

    (Korea University of Science and Technology
    Personalized Genomic Medicine Research Center, KRIBB)

  • Chan Yong Lee

    (Chungnam National University)

  • Kweon Yu

    (Disease Target Structure Research Center, KRIBB
    Korea Institute of Science and Technology
    Korea University of Science and Technology)

  • Nahum Sonenberg

    (McGill University, Montreal
    Rosalind and Morris Goodman Cancer Research Centre, Montreal)

  • Myung Hee Kim

    (Korea Research Institute of Bioscience and Biotechnology (KRIBB))

  • Sunghoon Kim

    (Seoul National University
    Seoul National University)

Abstract

A fundamental question in biology is how vertebrates evolved and differ from invertebrates, and little is known about differences in the regulation of translation in the two systems. Herein, we identify a threonyl-tRNA synthetase (TRS)-mediated translation initiation machinery that specifically interacts with eIF4E homologous protein, and forms machinery that is structurally analogous to the eIF4F-mediated translation initiation machinery via the recruitment of other translation initiation components. Biochemical and RNA immunoprecipitation analyses coupled to sequencing suggest that this machinery emerged as a gain-of-function event in the vertebrate lineage, and it positively regulates the translation of mRNAs required for vertebrate development. Collectively, our findings demonstrate that TRS evolved to regulate vertebrate translation initiation via its dual role as a scaffold for the assembly of initiation components and as a selector of target mRNAs. This work highlights the functional significance of aminoacyl-tRNA synthetases in the emergence and control of higher order organisms.

Suggested Citation

  • Seung Jae Jeong & Shinhye Park & Loi T. Nguyen & Jungwon Hwang & Eun-Young Lee & Hoi-Khoanh Giong & Jeong-Soo Lee & Ina Yoon & Ji-Hyun Lee & Jong Hyun Kim & Hoi Kyoung Kim & Doyeun Kim & Won Suk Yang , 2019. "A threonyl-tRNA synthetase-mediated translation initiation machinery," Nature Communications, Nature, vol. 10(1), pages 1-15, December.
  • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-09086-0
    DOI: 10.1038/s41467-019-09086-0
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    Cited by:

    1. Keith Siew & Kevin A. Nestler & Charlotte Nelson & Viola D’Ambrosio & Chutong Zhong & Zhongwang Li & Alessandra Grillo & Elizabeth R. Wan & Vaksha Patel & Eliah Overbey & JangKeun Kim & Sanghee Yun & , 2024. "Cosmic kidney disease: an integrated pan-omic, physiological and morphological study into spaceflight-induced renal dysfunction," Nature Communications, Nature, vol. 15(1), pages 1-20, December.

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