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Light sheet microscopy with acoustic sample confinement

Author

Listed:
  • Zhengyi Yang

    (University of St Andrews
    Harwell Science and Innovation Campus)

  • Katy L. H. Cole

    (University of Edinburgh)

  • Yongqiang Qiu

    (University of Glasgow
    Liverpool John Moores University)

  • Ildikó M. L. Somorjai

    (University of St Andrews
    University of St Andrews)

  • Philip Wijesinghe

    (University of St Andrews
    The University of Western Australia
    The University of Western Australia)

  • Jonathan Nylk

    (University of St Andrews)

  • Sandy Cochran

    (University of Glasgow)

  • Gabriel C. Spalding

    (Illinois Wesleyan University)

  • David A. Lyons

    (University of Edinburgh)

  • Kishan Dholakia

    (University of St Andrews)

Abstract

Contactless sample confinement would enable a whole host of new studies in developmental biology and neuroscience, in particular, when combined with long-term, wide-field optical imaging. To achieve this goal, we demonstrate a contactless acoustic gradient force trap for sample confinement in light sheet microscopy. Our approach allows the integration of real-time environmentally controlled experiments with wide-field low photo-toxic imaging, which we demonstrate on a variety of marine animal embryos and larvae. To illustrate the key advantages of our approach, we provide quantitative data for the dynamic response of the heartbeat of zebrafish larvae to verapamil and norepinephrine, which are known to affect cardiovascular function. Optical flow analysis allows us to explore the cardiac cycle of the zebrafish and determine the changes in contractile volume within the heart. Overcoming the restrictions of sample immobilisation and mounting can open up a broad range of studies, with real-time drug-based assays and biomechanical analyses.

Suggested Citation

  • Zhengyi Yang & Katy L. H. Cole & Yongqiang Qiu & Ildikó M. L. Somorjai & Philip Wijesinghe & Jonathan Nylk & Sandy Cochran & Gabriel C. Spalding & David A. Lyons & Kishan Dholakia, 2019. "Light sheet microscopy with acoustic sample confinement," Nature Communications, Nature, vol. 10(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-08514-5
    DOI: 10.1038/s41467-019-08514-5
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    Cited by:

    1. Mia Kvåle Løvmo & Shiyu Deng & Simon Moser & Rainer Leitgeb & Wolfgang Drexler & Monika Ritsch-Marte, 2024. "Ultrasound-induced reorientation for multi-angle optical coherence tomography," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    2. Jan Durrer & Prajwal Agrawal & Ali Ozgul & Stephan C. F. Neuhauss & Nitesh Nama & Daniel Ahmed, 2022. "A robot-assisted acoustofluidic end effector," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    3. Matthew Stein & Sam Keller & Yujie Luo & Ognjen Ilic, 2022. "Shaping contactless radiation forces through anomalous acoustic scattering," Nature Communications, Nature, vol. 13(1), pages 1-8, December.

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