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Prevalence of Enterovirus in Water Consumed in Rural Areas in a State in the Midwest Region of Brazil

Author

Listed:
  • Graziela Picciola Bordoni

    (Institute of Tropical Pathology and Public Health, Federal University of Goiás, 235 Street, Goiânia 74605-050, Brazil)

  • Lucas Candido Gonçalves Barbosa

    (Medicine College, Federal University of Goiás, 235 Street, Goiânia 74690-900, Brazil)

  • Thais Reis Oliveira

    (Medicine College, Federal University of Goiás, 235 Street, Goiânia 74690-900, Brazil)

  • Fernando Santos Lima

    (Institute of Tropical Pathology and Public Health, Federal University of Goiás, 235 Street, Goiânia 74605-050, Brazil)

  • Viviane Monteiro Goes

    (Paraná Institute of Molecular Biology (IBMP), Professor Algacyr Munhoz Mader Street, 3775—Industrial City of Curitiba, Curitiba 81350-010, Brazil)

  • Mariely Cordeiro Estrela

    (Paraná Institute of Molecular Biology (IBMP), Professor Algacyr Munhoz Mader Street, 3775—Industrial City of Curitiba, Curitiba 81350-010, Brazil)

  • Priscila Zanette de Souza

    (Paraná Institute of Molecular Biology (IBMP), Professor Algacyr Munhoz Mader Street, 3775—Industrial City of Curitiba, Curitiba 81350-010, Brazil)

  • Mônica de Oliveira Santos

    (Medicine College, Federal University of Goiás, 235 Street, Goiânia 74690-900, Brazil)

  • Guilherme Rocha Lino de Souza

    (Institute of Biological Sciences, Federal University of Goiás, Chácaras California Avenue, Goiânia 74690-612, Brazil)

  • José Daniel Gonçalves Vieira

    (Institute of Tropical Pathology and Public Health, Federal University of Goiás, 235 Street, Goiânia 74605-050, Brazil)

  • Paulo Sérgio Scalize

    (School of Civil Engineering, Federal University of Goiás, Q University Street, lt. 1488, bl. A, sl. 7, Goiânia 74605-010, Brazil)

  • Lilian Carla Carneiro

    (Institute of Tropical Pathology and Public Health, Federal University of Goiás, 235 Street, Goiânia 74605-050, Brazil)

Abstract

Individuals in rural communities often obtain water from surface and groundwater sources, where the microbial quality is often unknown. Enteric viruses are among the main pathogenic microorganisms responsible for waterborne disease outbreaks. Thus, the objective of this work was to search for enterovirus in water samples from 25 rural communities. For this, 160 water samples were collected. Detection and quantification of the enterovirus (EV) were performed through molecular tests using the two main amplification reagents for qPCR. The prevalence of EV was identified in 4.4% (7/160) of the samples when Sybr Green ® was used, all in groundwater sources. Additionally, EV was found in 9.7% of shallow tubular wells, 3.8% of deep tubular wells, 4.3% of shallow dug wells and 5.9% of spring water. When using TaqMan ® , there was no amplification of the EV cDNA. Conclusions: Sybr Green ® , being a more accessible reagent, has a greater predilection for molecular tests, but the study showed that Taqman ® could suffer less interference from environmental samples, resulting in more reliable values of viral quantification. In this context, the detection of EV in groundwater can help in monitoring the virus in this source, in addition to helping managers of these communities in decision making.

Suggested Citation

  • Graziela Picciola Bordoni & Lucas Candido Gonçalves Barbosa & Thais Reis Oliveira & Fernando Santos Lima & Viviane Monteiro Goes & Mariely Cordeiro Estrela & Priscila Zanette de Souza & Mônica de Oliv, 2023. "Prevalence of Enterovirus in Water Consumed in Rural Areas in a State in the Midwest Region of Brazil," Sustainability, MDPI, vol. 15(13), pages 1-20, June.
    • Handle: RePEc:gam:jsusta:v:15:y:2023:i:13:p:9886-:d:1176109
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    References listed on IDEAS

    as
    1. Xiaotong Wen & Huilie Zheng & Fang Yuan & Hui Zhu & Duyi Kuang & Zhiqiang Shen & Yuanan Lu & Zhaokang Yuan, 2019. "Comparative Study of Two Methods of Enteric Virus Detection and Enteric Virus Relationship with Bacterial Indicator in Poyang Lake, Jiangxi, China," IJERPH, MDPI, vol. 16(18), pages 1-15, September.
    2. Osvalda De Giglio & Giuseppina Caggiano & Francesco Bagordo & Giovanna Barbuti & Silvia Brigida & Federica Lugoli & Tiziana Grassi & Giuseppina La Rosa & Luca Lucentini & Vito Felice Uricchio & Antone, 2017. "Enteric Viruses and Fecal Bacteria Indicators to Assess Groundwater Quality and Suitability for Irrigation," IJERPH, MDPI, vol. 14(6), pages 1-13, May.
    3. Hítalo Tobias Lôbo Lopes & Luis Rodrigo Fernandes Baumann & Paulo Sérgio Scalize, 2023. "A Contamination Predictive Model for Escherichia coli in Rural Communities Dug Shallow Wells," Sustainability, MDPI, vol. 15(3), pages 1-16, January.
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