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Expressing OsiSAP8 , a Zinc-Finger Associated Protein Gene, Mitigates Stress Dynamics in Existing Elite Rice Varieties of the ‘Green Revolution’

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  • Subramanian Radhesh Krishnan

    (Department of Biotechnology, Alagappa University, Karaikudi 630004, Tamil Nadu, India
    Research and Development Centre, T. Stanes and Company Limited, Coimbatore 641018, Tamil Nadu, India.)

  • Pandiyan Muthuramalingam

    (Department of Biotechnology, Alagappa University, Karaikudi 630004, Tamil Nadu, India
    Department of Horticultural Science, Gyeongsang National University, Jinju 52727, Korea.)

  • Arumugam Mohana Priya

    (Department of Biotechnology, Alagappa University, Karaikudi 630004, Tamil Nadu, India)

  • Mani Iyer Prasanth

    (Department of Biotechnology, Alagappa University, Karaikudi 630004, Tamil Nadu, India
    Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand)

  • Krishnasamy Gopinath

    (Department of Bioinformatics, Alagappa University, Karaikudi 630004, Tamil Nadu, India
    Faculty of Medicine, Integrative Physiology and Pharmacology, Institute of Biomedicine, University of Turku, 20014 Turku, Finland.)

  • Chakravarthi Mohan

    (Department of Genetics and Evolution, Federal University of Sao Carlos, São Carlos 13565-905, Brazil
    Department of Agronomy, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611, USA)

  • Karthikeyan Muthusamy

    (Department of Bioinformatics, Alagappa University, Karaikudi 630004, Tamil Nadu, India)

  • Krishnaswamy Balamurugan

    (Department of Biotechnology, Alagappa University, Karaikudi 630004, Tamil Nadu, India)

  • Aditya Kumar Gupta

    (Dean School of Life Sciences, Department of Biotechnology, Central University of Rajasthan, Ajmer 305817, Rajasthan, India)

  • Manikandan Ramesh

    (Department of Biotechnology, Alagappa University, Karaikudi 630004, Tamil Nadu, India)

Abstract

Key message: Overexpression of OsiSAP8 driven by Port Ubi2.3 from Porteresia coarctata imparts drought and salinity stress tolerance in transgenic rice. Stress associated proteins (SAPs) possess the zinc-finger domains that are wildly evolving functional and conserved regions/factors in plants to combat abiotic stresses. In this study, the promoter region of OsiSAP8 , an intron-less, multiple stress inducible gene, was compared in silico with a strong constitutive promoter, Port Ubi2.3 . This resulted in developing rice, resistant to drought and salinity expressing OsiSAP8 promoted by Port Ubi2.3 . ( Porteresia coarctata ), through Agrobacterium -mediated transformation in the popular rice varieties, IR36 and IR64. Southern blot hybridization confirmed the integration of OsiSAP8 , and the T0 transgenic lines of IR36 and IR64 were evaluated for their drought and salinity tolerance. The IR36-T1 progenies showed an enhanced tolerance to water withhold stress compared to wild type and IR64-T1 progenies. Physiological parameters, such as the panicle weight, number of panicles, leaf wilting, and TBARS assay, showed the transgenic IR36 to be superior. The transgenic lines performed better with higher 80–95% relative leaf water content when subjected to drought for 14 days. Gene expression analysis of OsiSAP8 in IR36 T1 showed a 1.5-fold upregulation under mannitol stress. However, IR64 T1 showed a two-fold upregulation in NaCl stress. An enhanced drought and salinity stress tolerance in the transgenic IR36 cultivar through overexpression of OsiSAP8 was observed as it had a native copy of OsiSAP8 . This is perhaps the first study using a novel ubiquitin promoter ( Port Ubi2.3 ) to generate drought and salinity stress-tolerant transgenic rice. Thus, we report the overexpression of a rice gene ( OsiSAP8 ) by a rice promoter ( Port Ubi2.3 ) in rice (IR36) to resist drought and salinity.

Suggested Citation

  • Subramanian Radhesh Krishnan & Pandiyan Muthuramalingam & Arumugam Mohana Priya & Mani Iyer Prasanth & Krishnasamy Gopinath & Chakravarthi Mohan & Karthikeyan Muthusamy & Krishnaswamy Balamurugan & Ad, 2022. "Expressing OsiSAP8 , a Zinc-Finger Associated Protein Gene, Mitigates Stress Dynamics in Existing Elite Rice Varieties of the ‘Green Revolution’," Sustainability, MDPI, vol. 14(16), pages 1-29, August.
    • Handle: RePEc:gam:jsusta:v:14:y:2022:i:16:p:10174-:d:889699
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    References listed on IDEAS

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