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Harnessing the Power of Mutagenesis and Adaptive Laboratory Evolution for High Lipid Production by Oleaginous Microalgae and Yeasts

Author

Listed:
  • Neha Arora

    (Patel College of Global Sustainability, University of South Florida, 4202 E. Fowler Avenue, Tampa, FL 33620, USA)

  • Hong-Wei Yen

    (Department of Chemical and Materials Engineering, Tunghai University, Taichung City 407302, Taiwan)

  • George P. Philippidis

    (Patel College of Global Sustainability, University of South Florida, 4202 E. Fowler Avenue, Tampa, FL 33620, USA)

Abstract

Oleaginous microalgae and yeasts represent promising candidates for large-scale production of lipids, which can be utilized for production of drop-in biofuels, nutraceuticals, pigments, and cosmetics. However, low lipid productivity and costly downstream processing continue to hamper the commercial deployment of oleaginous microorganisms. Strain improvement can play an essential role in the development of such industrial microorganisms by increasing lipid production and hence reducing production costs. The main means of strain improvement are random mutagenesis, adaptive laboratory evolution (ALE), and rational genetic engineering. Among these, random mutagenesis and ALE are straight forward, low-cost, and do not require thorough knowledge of the microorganism’s genetic composition. This paper reviews available mutagenesis and ALE techniques and screening methods to effectively select for oleaginous microalgae and yeasts with enhanced lipid yield and understand the alterations caused to metabolic pathways, which could subsequently serve as the basis for further targeted genetic engineering.

Suggested Citation

  • Neha Arora & Hong-Wei Yen & George P. Philippidis, 2020. "Harnessing the Power of Mutagenesis and Adaptive Laboratory Evolution for High Lipid Production by Oleaginous Microalgae and Yeasts," Sustainability, MDPI, vol. 12(12), pages 1-27, June.
    • Handle: RePEc:gam:jsusta:v:12:y:2020:i:12:p:5125-:d:375447
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    References listed on IDEAS

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    1. Ju, Jung-Hyun & Ko, Dong-Jin & Heo, Sun-Yeon & Lee, Jong-Jea & Kim, Young-Min & Lee, Bong-Soo & Kim, Min-Soo & Kim, Chul-Ho & Seo, Jeong-Woo & Oh, Beak-Rock, 2020. "Regulation of lipid accumulation using nitrogen for microalgae lipid production in Schizochytrium sp. ABC101," Renewable Energy, Elsevier, vol. 153(C), pages 580-587.
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    Cited by:

    1. Thurakit, Theera & Pathom-aree, Wasu & Pumas, Chayakorn & Brocklehurst, Thanyanan Wannathong & Pekkoh, Jeeraporn & Srinuanpan, Sirasit, 2022. "High-efficiency production of biomass and biofuel under two-stage cultivation of a stable microalga Botryococcus braunii mutant generated by ethyl methanesulfonate-induced mutation," Renewable Energy, Elsevier, vol. 198(C), pages 176-188.
    2. Dar, Rouf Ahmad & Tsui, To-Hung & Zhang, Le & Tong, Yen Wah & Sharon, Sigal & Shoseyov, Oded & Liu, Ronghou, 2024. "Fermentation of organic wastes through oleaginous microorganisms for lipid production - Challenges and opportunities," Renewable and Sustainable Energy Reviews, Elsevier, vol. 195(C).
    3. Shubhanvit Mishra & Yi-Ju Liu & Chi-Shuo Chen & Da-Jeng Yao, 2021. "An Easily Accessible Microfluidic Chip for High-Throughput Microalgae Screening for Biofuel Production," Energies, MDPI, vol. 14(7), pages 1-10, March.
    4. Joseph Antony Sundarsingh Tensingh & Vijayalakshmi Shankar, 2022. "Sustainable Production of Biodiesel Using UV Mutagenesis as a Strategy to Enhance the Lipid Productivity in R. mucilaginosa," Sustainability, MDPI, vol. 14(15), pages 1-15, July.

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