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Investigation into the Effect of Molds in Grasses on Their Content of Low Molecular Mass Thiols

Author

Listed:
  • Jiri Skladanka

    (Department of Animal Nutrition and Forage Production, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic)

  • Vojtech Adam

    (Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic
    Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic)

  • Ondrej Zitka

    (Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic
    Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic
    Department of Veterinary Ecology and Environmental Protection, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Palackeho 1–3, CZ-612 42 Brno, Czech Republic)

  • Olga Krystofova

    (Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic)

  • Miroslava Beklova

    (Department of Veterinary Ecology and Environmental Protection, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Palackeho 1–3, CZ-612 42 Brno, Czech Republic)

  • Rene Kizek

    (Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic
    Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic)

  • Zdenek Havlicek

    (Department of Animal Morphology, Physiology and Genetics, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic)

  • Petr Slama

    (Department of Animal Morphology, Physiology and Genetics, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic)

  • Adam Nawrath

    (Department of Animal Nutrition and Forage Production, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic)

Abstract

The aim of this study was to investigate the effect of molds on levels of low molecular mass thiols in grasses. For this purpose, the three grass species Lolium perenne , Festulolium pabulare and Festulolium braunii were cultivated and sampled during four months, from June to September. The same species were also grown under controlled conditions. High-performance liquid chromatography with electrochemical detection was used for quantification of cysteine, reduced (GSH) and oxidized (GSSG) glutathione, and phytochelatins (PC2, PC3, PC4 and PC5). Data were statistically processed and analyzed. Thiols were present in all examined grass species. The effect of fungicide treatments applied under field conditions on the content of the evaluated thiols was shown to be insignificant. Species influenced ( p < 0.05) PC3 and GSSG content. F. pabulare , an intergeneric hybrid of drought- and fungi-resistant Festuca arundinacea , was comparable in PC3 content with L. perenne and F. braunii under field conditions. Under controlled conditions, however, F. pabulare had higher ( p < 0.05) PC3 content than did L. perenne and F. braunii . Under field conditions, differences between the evaluated species were recorded only in GSSG content, but only sampling in June was significant. F. pabulare had higher ( p < 0.05) GSSG content in June than did L. perenne and F. braunii .

Suggested Citation

  • Jiri Skladanka & Vojtech Adam & Ondrej Zitka & Olga Krystofova & Miroslava Beklova & Rene Kizek & Zdenek Havlicek & Petr Slama & Adam Nawrath, 2012. "Investigation into the Effect of Molds in Grasses on Their Content of Low Molecular Mass Thiols," IJERPH, MDPI, vol. 9(11), pages 1-17, October.
    • Handle: RePEc:gam:jijerp:v:9:y:2012:i:11:p:3789-3805:d:20928
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    Cited by:

    1. Miguel Angel Merlos Rodrigo & Natalia Cernei & Marketa Kominkova & Ondrej Zitka & Miroslava Beklova & Josef Zehnalek & Rene Kizek & Vojtech Adam, 2013. "Ion Exchange Chromatography and Mass Spectrometric Methods for Analysis of Cadmium-Phytochelatin (II) Complexes," IJERPH, MDPI, vol. 10(4), pages 1-8, March.

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