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Ion Exchange Chromatography and Mass Spectrometric Methods for Analysis of Cadmium-Phytochelatin (II) Complexes

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  • Miguel Angel Merlos Rodrigo

    (Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic
    Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic)

  • Natalia Cernei

    (Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic)

  • Marketa Kominkova

    (Department of Veterinary Ecology and Environmental Protection, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Palackeho 1-3, CZ-612 42 Brno, Czech Republic)

  • Ondrej Zitka

    (Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic
    Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic
    Department of Veterinary Ecology and Environmental Protection, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Palackeho 1-3, CZ-612 42 Brno, Czech Republic)

  • Miroslava Beklova

    (Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic
    Department of Veterinary Ecology and Environmental Protection, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Palackeho 1-3, CZ-612 42 Brno, Czech Republic)

  • Josef Zehnalek

    (Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic
    Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic)

  • Rene Kizek

    (Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic
    Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic
    Lead and Cadmium Initiatives, United Nations Environment Program, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic)

  • Vojtech Adam

    (Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic
    Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic)

Abstract

In this study, in vitro formed Cd-phytochelatin (PC2) complexes were characterized using ion exchange chromatography (IEC) and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. The ratio of both studied compounds as well as experimental conditions were optimized. The highest yield of the complex was observed under an applied concentration of 100 µg·mL −1 PC2 and 100 µg·mL −1 of CdCl 2 . The data obtained show that IEC in combination with MALDI-TOF is a reliable and fast method for the determination of these complexes.

Suggested Citation

  • Miguel Angel Merlos Rodrigo & Natalia Cernei & Marketa Kominkova & Ondrej Zitka & Miroslava Beklova & Josef Zehnalek & Rene Kizek & Vojtech Adam, 2013. "Ion Exchange Chromatography and Mass Spectrometric Methods for Analysis of Cadmium-Phytochelatin (II) Complexes," IJERPH, MDPI, vol. 10(4), pages 1-8, March.
    • Handle: RePEc:gam:jijerp:v:10:y:2013:i:4:p:1304-1311:d:24645
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    References listed on IDEAS

    as
    1. Olga Krystofova & Vojtech Adam & Petr Babula & Josef Zehnalek & Miroslava Beklova & Ladislav Havel & Rene Kizek, 2010. "Effects of Various Doses of Selenite on Stinging Nettle ( Urtica dioica L.)," IJERPH, MDPI, vol. 7(10), pages 1-12, October.
    2. Gloria Miller & Gregorio Begonia & Maria F. T. Begonia, 2011. "Selected Morphological Characteristics, Lead Uptake and Phytochelatin Synthesis by Coffeeweed ( Sesbania exaltata Raf.) Grown in Elevated Levels of Lead-Contaminated Soil," IJERPH, MDPI, vol. 8(6), pages 1-17, June.
    3. Jiri Skladanka & Vojtech Adam & Ondrej Zitka & Olga Krystofova & Miroslava Beklova & Rene Kizek & Zdenek Havlicek & Petr Slama & Adam Nawrath, 2012. "Investigation into the Effect of Molds in Grasses on Their Content of Low Molecular Mass Thiols," IJERPH, MDPI, vol. 9(11), pages 1-17, October.
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