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Prevalence and Subtype Distribution of Blastocystis Isolated from School-Aged Children in the Thai-Myanmar Border, Ratchaburi Province, Thailand

Author

Listed:
  • Amanee Abu

    (Department of Protozoology, Faculty of Tropical Medicine, Mahidol University, 420/6 Ratchawithi Road, Bangkok 10400, Thailand)

  • Chantira Sutthikornchai

    (Department of Protozoology, Faculty of Tropical Medicine, Mahidol University, 420/6 Ratchawithi Road, Bangkok 10400, Thailand)

  • Aongart Mahittikorn

    (Department of Protozoology, Faculty of Tropical Medicine, Mahidol University, 420/6 Ratchawithi Road, Bangkok 10400, Thailand)

  • Khuanchai Koompapong

    (Department of Protozoology, Faculty of Tropical Medicine, Mahidol University, 420/6 Ratchawithi Road, Bangkok 10400, Thailand)

  • Rachatawan Chiabchalard

    (Department of Protozoology, Faculty of Tropical Medicine, Mahidol University, 420/6 Ratchawithi Road, Bangkok 10400, Thailand)

  • Dumrongkiet Arthan

    (Department of Tropical Nutrition and Food Science, Faculty of Tropical Medicine, Mahidol University, 420/6 Ratchawithi Road, Bangkok 10400, Thailand)

  • Ngamphol Soonthornworasiri

    (Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, 420/6 Ratchawithi Road, Bangkok 10400, Thailand)

  • Supaluk Popruk

    (Department of Protozoology, Faculty of Tropical Medicine, Mahidol University, 420/6 Ratchawithi Road, Bangkok 10400, Thailand)

Abstract

Blastocystis is one of the most common enteric protozoa that inhabits the intestinal tract of humans and different animals. Moreover, it has a worldwide geographic distribution. Its main mode of transmission is via the fecal-oral route. At present, 26 subtypes are widely distributed across both humans and animals. The current study aimed to determine the prevalence and subtype distribution of Blastocystis among school-aged children living on the Thai-Myanmar border, Ratchaburi province, Thailand. In total, 508 samples were collected from children at six schools. The prevalence of Blastocystis infection was amplified and sequenced in the 600 bp barcode region of the small-subunit ribosomal RNA (SSU rRNA). The overall prevalence of Blastocystis infection was 3.35% (17/508). ST3 (11/17) was the most predominant subtype, followed by ST1 (5/17) and ST2 (1/17). A phylogenetic tree was constructed based on the Tamura92+G+I model using the maximum-likelihood algorithm. Surprisingly, all sequences of the ST3-positive samples were closely correlated with the cattle-derived sequence. Meanwhile, all sequences of the Blastocystis ST1-positive samples were closely correlated with the human-derived sequence. Nevertheless, further studies should be conducted to validate the zoonotic transmission of Blastocystis . Based on our findings, personal hygiene and sanitation should be improved to promote better health in children in this area.

Suggested Citation

  • Amanee Abu & Chantira Sutthikornchai & Aongart Mahittikorn & Khuanchai Koompapong & Rachatawan Chiabchalard & Dumrongkiet Arthan & Ngamphol Soonthornworasiri & Supaluk Popruk, 2022. "Prevalence and Subtype Distribution of Blastocystis Isolated from School-Aged Children in the Thai-Myanmar Border, Ratchaburi Province, Thailand," IJERPH, MDPI, vol. 20(1), pages 1-9, December.
    • Handle: RePEc:gam:jijerp:v:20:y:2022:i:1:p:204-:d:1012704
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    References listed on IDEAS

    as
    1. Noppon Popruk & Satakamol Prasongwattana & Aongart Mahittikorn & Attakorn Palasuwan & Supaluk Popruk & Duangdao Palasuwan, 2020. "Prevalence and Subtype Distribution of Blastocystis Infection in Patients with Diabetes Mellitus in Thailand," IJERPH, MDPI, vol. 17(23), pages 1-12, November.
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