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Effect of Humin and Chemical Factors on CO 2 -Fixing Acetogenesis and Methanogenesis

Author

Listed:
  • Biec Nhu Ha

    (Department of Civil Engineering, Graduate School of Engineering, Nagoya University, Chikusa, Nagoya 464-8603, Japan)

  • Duyen Minh Pham

    (Institute of Materials and Systems for Sustainability, Nagoya University, Chikusa, Nagoya 464-8603, Japan)

  • Takuya Kasai

    (Department of Civil Engineering, Graduate School of Engineering, Nagoya University, Chikusa, Nagoya 464-8603, Japan
    Institute of Materials and Systems for Sustainability, Nagoya University, Chikusa, Nagoya 464-8603, Japan)

  • Takanori Awata

    (Graduate School of Engineering, Osaka Institute of Technology, Osaka 535-8585, Japan)

  • Arata Katayama

    (Department of Civil Engineering, Graduate School of Engineering, Nagoya University, Chikusa, Nagoya 464-8603, Japan
    Institute of Materials and Systems for Sustainability, Nagoya University, Chikusa, Nagoya 464-8603, Japan)

Abstract

Acetogenesis and methanogenesis have attracted attention as CO 2 -fixing reactions. Humin, a humic substance insoluble at any pH, has been found to assist CO 2 -fixing acetogenesis as the sole electron donor. Here, using two CO 2 -fixing consortia with acetogenic and methanogenic activities, the effect of various parameters on these activities was examined. One consortium utilized humin and hydrogen (H 2 ) as electron donors for acetogenesis, either separately or simultaneously, but with a preference for the electron use from humin. The acetogenic activity was accelerated 14 times by FeS at 0.2 g/L as the optimal concentration, while being inhibited by MgSO 4 at concentration above 0.02 g/L and by NaCl at concentrations higher than 6 g/L. Another consortium did not utilize humin but H 2 as electron donor, suggesting that humin was not a universal electron donor for acetogenesis. For methanogenesis, both consortia did not utilize extracellular electrons from humin unless H 2 was present. The methanogenesis was promoted by FeS at 0.2 g/L or higher concentrations, especially without humin, and with NaCl at 2 g/L or higher concentrations regardless of the presence of humin, while no significant effect was observed with MgSO 4 . Comparative sequence analysis of partial 16S rRNA genes suggested that minor groups were the humin-utilizing acetogens in the consortium dominated by Clostridia , while Methanobacterium was the methanogen utilizing humin with H 2 .

Suggested Citation

  • Biec Nhu Ha & Duyen Minh Pham & Takuya Kasai & Takanori Awata & Arata Katayama, 2022. "Effect of Humin and Chemical Factors on CO 2 -Fixing Acetogenesis and Methanogenesis," IJERPH, MDPI, vol. 19(5), pages 1-16, February.
  • Handle: RePEc:gam:jijerp:v:19:y:2022:i:5:p:2546-:d:755935
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    References listed on IDEAS

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    1. Mahasweta Laskar & Takuya Kasai & Takanori Awata & Arata Katayama, 2020. "Humin Assists Reductive Acetogenesis in Absence of Other External Electron Donor," IJERPH, MDPI, vol. 17(12), pages 1-13, June.
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    Cited by:

    1. Chen, Dan & Pei, Haoyi & Zhou, Ningli & Xiao, Zhixing, 2024. "CO2 reduction to CH4 by Methanosarcina barkeri and a mixed methanogenic culture using humin as sole electron donor," Energy, Elsevier, vol. 294(C).

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