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Metabolic and Proteomic Profiles Reveal the Response of the ASD-Associated Resistant Strain 6-1 of Lactobacillus plantarum to Propionic Acid

Author

Listed:
  • Rongrong Yu

    (College of Education, Zhejiang University of Technology, Hangzhou 310032, China)

  • Muchen Zhang

    (Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China)

  • Temoor Ahmed

    (Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China)

  • Zhifeng Wu

    (Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China)

  • Luqiong Lv

    (Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China)

  • Guoling Zhou

    (Hangzhou Seventh People’s Hospital (HSPH), Hangzhou 310013, China)

  • Bin Li

    (Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China)

Abstract

Autism spectrum disorder (ASD) seriously affects children’s health. In our previous study, we isolated and identified a bacterium ( Lactobacillus plantarum strain 6-1) that is resistant to propionic acid (PA), which has been reported to play a significant role in the formation of ASD. In order to elucidate the mechanism of the resistance to PA, this study investigated the change in the metabolic and proteomic profile of L. plantarum strain 6-1 in the presence and absence of PA. The results show that 967 and 1078 proteins were specifically identified in the absence and the presence of PA, respectively, while 616 proteins were found under both conditions. Gene ontology enrichment analysis of 130 differentially expressed proteins accumulated in the presence and absence of PA indicated that most of the proteins belong to biological processes, cellular components, and molecular functions. Pathway enrichment analysis showed a great reduction in the metabolic pathway-related proteins when this resistant bacterium was exposed to PA compared to the control. Furthermore, there was an obvious difference in protein–protein interaction networks in the presence and the absence of propionic acid. In addition, there was a change in the metabolic profile of L. plantarum strain 6-1 when this bacterium was exposed to PA compared to the control, while six peaks at 696.46, 1543.022, 1905.241, 2004.277, 2037.374, and 2069.348 m / z disappeared. Overall, the results could help us to understand the mechanism of the resistance of gut bacteria to PA, which will provide a new insight for us to use PA-resistant bacteria to prevent the development of ASD in children.

Suggested Citation

  • Rongrong Yu & Muchen Zhang & Temoor Ahmed & Zhifeng Wu & Luqiong Lv & Guoling Zhou & Bin Li, 2022. "Metabolic and Proteomic Profiles Reveal the Response of the ASD-Associated Resistant Strain 6-1 of Lactobacillus plantarum to Propionic Acid," IJERPH, MDPI, vol. 19(24), pages 1-15, December.
    • Handle: RePEc:gam:jijerp:v:19:y:2022:i:24:p:17020-:d:1007338
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    References listed on IDEAS

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    1. Cassandra Willyard, 2021. "How gut microbes could drive brain disorders," Nature, Nature, vol. 590(7844), pages 22-25, February.
    2. Shih-Chen Fu & Chung-Han Lee & Hsiuying Wang, 2021. "Exploring the Association of Autism Spectrum Disorders and Constipation through Analysis of the Gut Microbiome," IJERPH, MDPI, vol. 18(2), pages 1-13, January.
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