IDEAS home Printed from https://ideas.repec.org/a/gam/jijerp/v18y2021i22p12168-d683552.html
   My bibliography  Save this article

Sex Steroid Regulation of Oxidative Stress in Bone Cells: An In Vitro Study

Author

Listed:
  • Valeria Sibilia

    (Department of Medical Biotechnology and Translational Medicine, Università degli Studi di Milano, 20129 Milano, Italy)

  • Daniele Bottai

    (Department of Health Sciences, Università degli Studi di Milano, 20142 Milano, Italy)

  • Roberto Maggi

    (Department of Pharmaceutical Sciences, Università degli Studi di Milano, 20133 Milano, Italy)

  • Francesca Pagani

    (Department of Medical Biotechnology and Translational Medicine, Università degli Studi di Milano, 20129 Milano, Italy)

  • Raffaella Chiaramonte

    (Department of Health Sciences, Università degli Studi di Milano, 20142 Milano, Italy)

  • Domenica Giannandrea

    (Department of Health Sciences, Università degli Studi di Milano, 20142 Milano, Italy)

  • Valentina Citro

    (Department of Health Sciences, Università degli Studi di Milano, 20142 Milano, Italy)

  • Natalia Platonova

    (Department of Health Sciences, Università degli Studi di Milano, 20142 Milano, Italy)

  • Lavinia Casati

    (Department of Health Sciences, Università degli Studi di Milano, 20142 Milano, Italy)

Abstract

Environmental stimuli, including sex hormones and oxidative stress (OS), affect bone balance, modifying the epigenetic profiles of key osteogenic genes. Nonetheless, the interplay between sex steroids, epigenome and OS has yet be fully elucidated. This paper aims to study in vitro the role of sex steroids in OS-induced alteration in bone cells’ homeostasis, and to assess the possible contribution of epigenetic modifications. Toward this purpose, osteoblast (MC3T3-E1) and osteocyte (MLOY-4) cell lines were exposed to two different sources of free oxygen radicals, i.e., tert-butyl hydroperoxide and dexamethasone, and the protective effect of pre-treatment with androgens and estrogens was evaluated. In particular, we analyzed parameters that reflect bone cell homeostasis such as cell viability, cell migration, transcriptomic profile, transcriptional activity, and epigenetic signature. Our findings indicate that estrogens and androgens counteract OS effects. Using partially overlapping strategies, they reduce OS outcomes regarding cell viability, cell migration, the transcriptomic profile of gene families involved in bone remodeling, and epigenetic profile, i.e., H3K4me3 level. Additionally, we demonstrated that the protective effect of steroids against OS on bone homeostasis is partially mediated by the Akt pathway. Overall, these results suggest that the hormonal milieu may influence the mechanisms of age-related bone disease.

Suggested Citation

  • Valeria Sibilia & Daniele Bottai & Roberto Maggi & Francesca Pagani & Raffaella Chiaramonte & Domenica Giannandrea & Valentina Citro & Natalia Platonova & Lavinia Casati, 2021. "Sex Steroid Regulation of Oxidative Stress in Bone Cells: An In Vitro Study," IJERPH, MDPI, vol. 18(22), pages 1-23, November.
    • Handle: RePEc:gam:jijerp:v:18:y:2021:i:22:p:12168-:d:683552
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/1660-4601/18/22/12168/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/1660-4601/18/22/12168/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Colin Jamora & Ramanuj DasGupta & Pawel Kocieniewski & Elaine Fuchs, 2003. "Erratum: Links between signal transduction, transcription and adhesion in epithelial bud development," Nature, Nature, vol. 424(6951), pages 974-974, August.
    2. Colin Jamora & Ramanuj DasGupta & Pawel Kocieniewski & Elaine Fuchs, 2003. "Links between signal transduction, transcription and adhesion in epithelial bud development," Nature, Nature, vol. 422(6929), pages 317-322, March.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Cummings, F.W, 2004. "A model of morphogenesis," Physica A: Statistical Mechanics and its Applications, Elsevier, vol. 339(3), pages 531-547.

    More about this item

    Keywords

    bone; steroids milieu; histone modification; oxidative stress; H3K4me3; H4 acetylation;
    All these keywords.

    JEL classification:

    • H4 - Public Economics - - Publicly Provided Goods

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jijerp:v:18:y:2021:i:22:p:12168-:d:683552. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.