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Mitochondrial Impairment Induced by Sub-Chronic Exposure to Multi-Walled Carbon Nanotubes

Author

Listed:
  • Giuseppa Visalli

    (Department of Biomedical and Dental Sciences and Morphofunctional Imaging. University of Messina, 98125 Messina, Italy)

  • Alessio Facciolà

    (Department of Clinical and Experimental Medicine, Unit of Infectious Diseases, University of Messina, 98125 Messina, Italy)

  • Monica Currò

    (Department of Biomedical and Dental Sciences and Morphofunctional Imaging. University of Messina, 98125 Messina, Italy)

  • Pasqualina Laganà

    (Department of Biomedical and Dental Sciences and Morphofunctional Imaging. University of Messina, 98125 Messina, Italy)

  • Vincenza La Fauci

    (Department of Biomedical and Dental Sciences and Morphofunctional Imaging. University of Messina, 98125 Messina, Italy)

  • Daniela Iannazzo

    (Department of Electronic Engineering, Industrial Chemistry and Engineering, University of Messina, 98125 Messina, Italy)

  • Alessandro Pistone

    (Department of Electronic Engineering, Industrial Chemistry and Engineering, University of Messina, 98125 Messina, Italy)

  • Angela Di Pietro

    (Department of Biomedical and Dental Sciences and Morphofunctional Imaging. University of Messina, 98125 Messina, Italy)

Abstract

Human exposure to carbon nanotubes (CNTs) can cause health issues due to their chemical-physical features and biological interactions. These nanostructures cause oxidative stress, also due to endogenous reactive oxygen species (ROS) production, which increases following mitochondrial impairment. The aim of this in vitro study was to assess the health effects, due to mitochondrial dysfunction, caused by a sub-chronic exposure to a non-acutely toxic dose of multi walled CNTs (raw and functionalised). The A549 cells were exposed to multi-walled carbon nanotubes (MWCNTs) (2 µg mL −1 ) for 36 days. Periodically, cellular dehydrogenases, pyruvate dehydrogenase kinase 1 (PDK1), cytochrome c release, permeability transition pore (mPTP) opening, transmembrane potential (Δψ m), apoptotic cells, and intracellular ROS were measured. The results, compared to untreated cells and to positive control formed by cells treated with MWCNTs (20 µg mL −1 ), highlighted the efficiency of homeostasis to counteract ROS overproduction, but a restitutio ad integrum of mitochondrial functionality was not observed. Despite the tendency to restore, the mitochondrial impairment persisted. Overall, the results underlined the tissue damage that can arise following sub-chronic exposure to MWCNTs.

Suggested Citation

  • Giuseppa Visalli & Alessio Facciolà & Monica Currò & Pasqualina Laganà & Vincenza La Fauci & Daniela Iannazzo & Alessandro Pistone & Angela Di Pietro, 2019. "Mitochondrial Impairment Induced by Sub-Chronic Exposure to Multi-Walled Carbon Nanotubes," IJERPH, MDPI, vol. 16(5), pages 1-13, March.
    • Handle: RePEc:gam:jijerp:v:16:y:2019:i:5:p:792-:d:210924
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    Citations

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    Cited by:

    1. Alessio Facciolà & Giuseppa Visalli & Marianna Pruiti Ciarello & Angela Di Pietro, 2021. "Newly Emerging Airborne Pollutants: Current Knowledge of Health Impact of Micro and Nanoplastics," IJERPH, MDPI, vol. 18(6), pages 1-17, March.
    2. Pasqualina Laganà & Giuseppa Visalli & Alessio Facciolà & Marianna Pruiti Ciarello & Antonio Laganà & Daniela Iannazzo & Angela Di Pietro, 2021. "Is the Antibacterial Activity of Multi-Walled Carbon Nanotubes (MWCNTs) Related to Antibiotic Resistance? An Assessment in Clinical Isolates," IJERPH, MDPI, vol. 18(17), pages 1-11, September.

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