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Characterization of Aerobic Denitrifying Bacterium Pseudomonas mendocina Strain GL6 and Its Potential Application in Wastewater Treatment Plant Effluent

Author

Listed:
  • Wen Zhang

    (State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, China)

  • Cheng Yan

    (State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, China)

  • Jianing Shen

    (State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, China)

  • Ruping Wei

    (State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, China)

  • Yan Gao

    (State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, China)

  • Aijun Miao

    (State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, China)

  • Lin Xiao

    (State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, China)

  • Liuyan Yang

    (State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, China)

Abstract

To remove nitrate in wastewater treatment plant effluent, an aerobic denitrifier was newly isolated from the surface flow constructed wetland and identified as Pseudomonas mendocina strain GL6. It exhibited efficient aerobic denitrification ability, with the nitrate removal rate of 6.61 mg (N)·L −1 ·h −1 . Sequence amplification indicated that the denitrification genes napA , nirK , norB , and nosZ were present in strain GL6. Nitrogen balance analysis revealed that approximately 74.5% of the initial nitrogen was removed as gas products. In addition, the response surface methodology experiments showed that the maximum removal of total nitrogen occurred at pH 7.76, C/N ratio of 11.2, temperature of 27.8 °C, and with shaking at 133 rpm. Furthermore, under the optimized cultivation condition, strain GL6 was added into wastewater treatment plant effluent and the removal rates of nitrate nitrogen and total nitrogen reached 95.6% and 73.6%, respectively. Thus, P. mendocina strain GL6 has high denitrification potential for deep improvement of effluent quality.

Suggested Citation

  • Wen Zhang & Cheng Yan & Jianing Shen & Ruping Wei & Yan Gao & Aijun Miao & Lin Xiao & Liuyan Yang, 2019. "Characterization of Aerobic Denitrifying Bacterium Pseudomonas mendocina Strain GL6 and Its Potential Application in Wastewater Treatment Plant Effluent," IJERPH, MDPI, vol. 16(3), pages 1-14, January.
    • Handle: RePEc:gam:jijerp:v:16:y:2019:i:3:p:364-:d:201385
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    References listed on IDEAS

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    1. Yahong Han & Shan Qiu & Hongyun Zeng & Fang Ma & Jue Wang & Yilun Qiu & Xuedi An, 2018. "Short-Term Effects of Tourmaline on Nitrogen Removals and Microbial Communities in a Sequencing Batch Reactor at Low Temperatures," IJERPH, MDPI, vol. 15(6), pages 1-12, June.
    2. Jiaxun Guo & Lachun Wang & Xiya Guo & Gengmao Zhao & Jiancai Deng & Chunfen Zeng, 2018. "Spatio-Temporal Differences in Nitrogen Reduction Rates under Biotic and Abiotic Processes in River Water of the Taihu Basin, China," IJERPH, MDPI, vol. 15(11), pages 1-17, November.
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    Cited by:

    1. Jilong Ren & Chenzheng Wei & Hongjing Ma & Mingyun Dai & Jize Fan & Ying Liu & Yinghai Wu & Rui Han, 2019. "The Nitrogen-Removal Efficiency of a Novel High-Efficiency Salt-Tolerant Aerobic Denitrifier, Halomonas Alkaliphile HRL-9, Isolated from a Seawater Biofilter," IJERPH, MDPI, vol. 16(22), pages 1-13, November.

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