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The Nitrogen-Removal Efficiency of a Novel High-Efficiency Salt-Tolerant Aerobic Denitrifier, Halomonas Alkaliphile HRL-9, Isolated from a Seawater Biofilter

Author

Listed:
  • Jilong Ren

    (School of Marine Technology and Environment, Dalian Ocean University, Dalian 116023, China
    Key Laboratory of Environment Controlled Aquaculture, Ministry of Education, Dalian Ocean University, 52 Heishijiao Street, Dalian 116023, China)

  • Chenzheng Wei

    (School of Marine Technology and Environment, Dalian Ocean University, Dalian 116023, China
    Key Laboratory of Environment Controlled Aquaculture, Ministry of Education, Dalian Ocean University, 52 Heishijiao Street, Dalian 116023, China)

  • Hongjing Ma

    (School of Marine Technology and Environment, Dalian Ocean University, Dalian 116023, China
    Key Laboratory of Environment Controlled Aquaculture, Ministry of Education, Dalian Ocean University, 52 Heishijiao Street, Dalian 116023, China)

  • Mingyun Dai

    (School of Marine Technology and Environment, Dalian Ocean University, Dalian 116023, China
    Key Laboratory of Environment Controlled Aquaculture, Ministry of Education, Dalian Ocean University, 52 Heishijiao Street, Dalian 116023, China)

  • Jize Fan

    (School of Marine Technology and Environment, Dalian Ocean University, Dalian 116023, China
    Key Laboratory of Environment Controlled Aquaculture, Ministry of Education, Dalian Ocean University, 52 Heishijiao Street, Dalian 116023, China)

  • Ying Liu

    (School of Marine Technology and Environment, Dalian Ocean University, Dalian 116023, China
    Key Laboratory of Environment Controlled Aquaculture, Ministry of Education, Dalian Ocean University, 52 Heishijiao Street, Dalian 116023, China)

  • Yinghai Wu

    (College of Marine and Civil Engineering, Dalian Ocean University, Dalian 116023, China
    South China Institute of Environmental Science, Ministry of Ecology and Environment, Guangzhou 510655, China)

  • Rui Han

    (School of Marine Technology and Environment, Dalian Ocean University, Dalian 116023, China
    Key Laboratory of Environment Controlled Aquaculture, Ministry of Education, Dalian Ocean University, 52 Heishijiao Street, Dalian 116023, China)

Abstract

Aerobic denitrification microbes have great potential to solve the problem of NO 3 − -N accumulation in industrialized recirculating aquaculture systems (RASs). A novel salt-tolerant aerobic denitrifier was isolated from a marine recirculating aquaculture system (RAS) and identified as Halomonas alkaliphile HRL-9. Its aerobic denitrification performance in different dissolved oxygen concentrations, temperatures, and C/N ratios was studied. Investigations into nitrogen balance and nitrate reductase genes ( napA and narG ) were also carried out. The results showed that the optimal conditions for nitrate removal were temperature of 30 °C, a shaking speed of 150 rpm, and a C/N ratio of 10. For nitrate nitrogen (NO 3 − -N) (initial concentration 101.8 mg·L −1 ), the sole nitrogen source of the growth of HRL-9, the maximum NO 3 − -N removal efficiency reached 98.0% after 24 h and the maximum total nitrogen removal efficiency was 77.3% after 48 h. Nitrogen balance analysis showed that 21.7% of NO 3 − -N was converted into intracellular nitrogen, 3.3% of NO 3 − -N was converted into other nitrification products (i.e., nitrous nitrogen, ammonium nitrogen, and organic nitrogen), and 74.5% of NO 3 − -N might be converted to gaseous products. The identification of functional genes confirmed the existence of the napA gene in strain HRL-9, but no narG gene was found. These results confirm that the aerobic denitrification strain, Halomonas alkaliphile HRL-9, which has excellent aerobic denitrification abilities, can also help us understand the microbiological mechanism and transformation pathway of aerobic denitrification in RASs.

Suggested Citation

  • Jilong Ren & Chenzheng Wei & Hongjing Ma & Mingyun Dai & Jize Fan & Ying Liu & Yinghai Wu & Rui Han, 2019. "The Nitrogen-Removal Efficiency of a Novel High-Efficiency Salt-Tolerant Aerobic Denitrifier, Halomonas Alkaliphile HRL-9, Isolated from a Seawater Biofilter," IJERPH, MDPI, vol. 16(22), pages 1-13, November.
    • Handle: RePEc:gam:jijerp:v:16:y:2019:i:22:p:4451-:d:286346
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    References listed on IDEAS

    as
    1. Hanyue Wang & Tong Wang & Shangye Yang & Xueqing Liu & Liqing Kou & Tinglin Huang & Gang Wen, 2019. "Nitrogen Removal in Oligotrophic Reservoir Water by a Mixed Aerobic Denitrifying Consortium: Influencing Factors and Immobilization Effects," IJERPH, MDPI, vol. 16(4), pages 1-15, February.
    2. Wen Zhang & Cheng Yan & Jianing Shen & Ruping Wei & Yan Gao & Aijun Miao & Lin Xiao & Liuyan Yang, 2019. "Characterization of Aerobic Denitrifying Bacterium Pseudomonas mendocina Strain GL6 and Its Potential Application in Wastewater Treatment Plant Effluent," IJERPH, MDPI, vol. 16(3), pages 1-14, January.
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    Cited by:

    1. Yinghai Wu & Xinyu Rong & Cuiya Zhang & Renduo Zhang & Tao He & Yunjun Yu & Zhuangming Zhao & Jing Yang & Rui Han, 2020. "Response of the Intertidal Microbial Community Structure and Metabolic Profiles to Zinc Oxide Nanoparticle Exposure," IJERPH, MDPI, vol. 17(7), pages 1-15, March.

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