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Study on the In Vitro Activity of Five Disinfectants against Nosocomial Bacteria

Author

Listed:
  • Maria Teresa Montagna

    (Department of Biomedical Science and Human Oncology, University of Bari “Aldo Moro”, Piazza G. Cesare 11, 70124 Bari, Italy)

  • Francesco Triggiano

    (Department of Biomedical Science and Human Oncology, University of Bari “Aldo Moro”, Piazza G. Cesare 11, 70124 Bari, Italy)

  • Giovanna Barbuti

    (Department of Biomedical Science and Human Oncology, University of Bari “Aldo Moro”, Piazza G. Cesare 11, 70124 Bari, Italy)

  • Nicola Bartolomeo

    (Department of Biomedical Science and Human Oncology, University of Bari “Aldo Moro”, Piazza G. Cesare 11, 70124 Bari, Italy)

  • Osvalda De Giglio

    (Department of Biomedical Science and Human Oncology, University of Bari “Aldo Moro”, Piazza G. Cesare 11, 70124 Bari, Italy)

  • Giusy Diella

    (Department of Biomedical Science and Human Oncology, University of Bari “Aldo Moro”, Piazza G. Cesare 11, 70124 Bari, Italy)

  • Marco Lopuzzo

    (Department of Biomedical Science and Human Oncology, University of Bari “Aldo Moro”, Piazza G. Cesare 11, 70124 Bari, Italy)

  • Serafina Rutigliano

    (Department of Biomedical Science and Human Oncology, University of Bari “Aldo Moro”, Piazza G. Cesare 11, 70124 Bari, Italy)

  • Gabriella Serio

    (Department of Biomedical Science and Human Oncology, University of Bari “Aldo Moro”, Piazza G. Cesare 11, 70124 Bari, Italy)

  • Giuseppina Caggiano

    (Department of Biomedical Science and Human Oncology, University of Bari “Aldo Moro”, Piazza G. Cesare 11, 70124 Bari, Italy)

Abstract

Nosocomial infections cause significant morbidity and mortality worldwide, and the pathogenic organisms responsible for such infections can develop resistance to antimicrobial agents. Understanding the activity of disinfectants against clinical and environmental bacterial isolates is therefore crucial. We analysed the in vitro activity of five antimicrobial products (phenolic compounds, didecyldimethylammonium chloride (DDAC), sodium hypochlorite, isopropanol + ammonium compounds (IACs), hydrogen peroxide) against 187 bacterial strains comprising clinical isolates, as well as 30 environmental isolates of Pseudomonas aeruginosa from hospital water samples. Disk diffusion assays were employed to assess antimicrobial activity. Hydrogen peroxide was significantly more active ( p < 0.0001) than the other disinfectants against all P. aeruginosa , Klebsiella pneumoniae , Enterococcus faecalis and Staphylococcus aureus strains. It was also the only disinfectant with activity against both clinical and environmental strains of P. aeruginosa . DDAC and IAC-based disinfectants were ineffective against Gram-negative strains, but showed significant activity (particularly IACs, p < 0.0001) against the Gram-positive strains. Compared with IACs, DDAC was significantly more active on E. faecalis and less active on S. aureus ( p < 0.0001). Sodium hypochlorite and phenol compounds, by contrast, were inactive against all bacterial strains. The development of disinfection procedures that are effective against all microorganisms is essential for limiting the spread of nosocomial infections.

Suggested Citation

  • Maria Teresa Montagna & Francesco Triggiano & Giovanna Barbuti & Nicola Bartolomeo & Osvalda De Giglio & Giusy Diella & Marco Lopuzzo & Serafina Rutigliano & Gabriella Serio & Giuseppina Caggiano, 2019. "Study on the In Vitro Activity of Five Disinfectants against Nosocomial Bacteria," IJERPH, MDPI, vol. 16(11), pages 1-9, May.
    • Handle: RePEc:gam:jijerp:v:16:y:2019:i:11:p:1895-:d:235213
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    Citations

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    Cited by:

    1. Giuseppina Caggiano & Francesco Triggiano & Giusy Diella & Francesca Apollonio & Marco Lopuzzo & Adriana Mosca & Stefania Stolfa & Carlo Pazzani & Marta Oliva & Carla Calia & Nicola Laforgia & Lidia D, 2021. "A Possible Outbreak by Serratia marcescens : Genetic Relatedness between Clinical and Environmental Strains," IJERPH, MDPI, vol. 18(18), pages 1-9, September.
    2. Francesco Triggiano & Giuseppina Caggiano & Marco Lopuzzo & Giusy Diella & Francesca Apollonio & Fabrizio Fasano & Maria Teresa Montagna, 2022. "No-Touch Automated Disinfection System Based on Hydrogen Peroxide and Ethyl Alcohol Aerosols for Use in Healthcare Environments," IJERPH, MDPI, vol. 19(8), pages 1-6, April.
    3. Giuseppina Di Martino & Salvatore Pasqua & Bruno Douradinha & Francesco Monaco & Chiara Di Bartolo & Pier Giulio Conaldi & Danilo D’Apolito, 2021. "Efficacy of Three Commercial Disinfectants in Reducing Microbial Surfaces’ Contaminations of Pharmaceuticals Hospital Facilities," IJERPH, MDPI, vol. 18(2), pages 1-11, January.

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