IDEAS home Printed from https://ideas.repec.org/a/gam/jijerp/v15y2018i6p1247-d152080.html
   My bibliography  Save this article

Antibiotic Resistance Characterization of Environmental E. coli Isolated from River Mula-Mutha, Pune District, India

Author

Listed:
  • Rutuja Dhawde

    (The Foundation for Medical Research, 84A, R.G. Thadani Marg, Worli, Mumbai 400 018, India)

  • Ragini Macaden

    (St Johns Research Institute, 100 Feet Rd, John Nagar, Koramangala, Bangalore 560 034, India)

  • Dhananjaya Saranath

    (Cancer Patients Aid Association (CPAA), Sumer Kendra, Mumbai 400 0018, India)

  • Kayzad Nilgiriwala

    (The Foundation for Medical Research, 84A, R.G. Thadani Marg, Worli, Mumbai 400 018, India)

  • Appasaheb Ghadge

    (The Foundation for Research in Community Health, Pune 411007, India)

  • Tannaz Birdi

    (The Foundation for Medical Research, 84A, R.G. Thadani Marg, Worli, Mumbai 400 018, India)

Abstract

In the current study, ceftazidime- and ciprofloxacin-resistant—or dual drug-resistant (DDR)— E. coli were isolated from river Mula-Mutha, which flows through rural Pune district and Pune city. The DDR E. coli were further examined for antibiotic resistance to six additional antibiotics. The study also included detection of genes responsible for ceftazidime and ciprofloxacin resistance and vectors for horizontal gene transfer. Twenty-eight percent of the identified DDR E. coli were resistant to more than six antibiotics, with 12% being resistant to all eight antibiotics tested. Quinolone resistance was determined through the detection of qnrA , qnrB , qnrS and oqxA genes, whereas cephalosporin resistance was confirmed through detection of TEM, CTX-M-15, CTX-M-27 and SHV genes. Out of 219 DDR E. coli , 8.2% were qnrS positive and 0.4% were qnrB positive. Percentage of isolates positive for the TEM, CTX-M-15 and CTX-M-27 genes were 32%, 46% and 0.9%, respectively. None of the DDR E. coli tested carried the qnrA , SHV and oqxA genes. Percentage of DDR E. coli carrying Class 1 and 2 integrons (mobile genetic elements) were 47% and 8%, respectively. The results showed that antibiotic resistance genes (ARGs) and integrons were present in the E. coli isolated from the river at points adjoining and downstream of Pune city.

Suggested Citation

  • Rutuja Dhawde & Ragini Macaden & Dhananjaya Saranath & Kayzad Nilgiriwala & Appasaheb Ghadge & Tannaz Birdi, 2018. "Antibiotic Resistance Characterization of Environmental E. coli Isolated from River Mula-Mutha, Pune District, India," IJERPH, MDPI, vol. 15(6), pages 1-15, June.
  • Handle: RePEc:gam:jijerp:v:15:y:2018:i:6:p:1247-:d:152080
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/1660-4601/15/6/1247/pdf
    Download Restriction: no

    File URL: https://www.mdpi.com/1660-4601/15/6/1247/
    Download Restriction: no
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Nada Hanna & Manju Purohit & Vishal Diwan & Salesh P. Chandran & Emilia Riggi & Vivek Parashar & Ashok J. Tamhankar & Cecilia Stålsby Lundborg, 2020. "Monitoring of Water Quality, Antibiotic Residues, and Antibiotic-Resistant Escherichia coli in the Kshipra River in India over a 3-Year Period," IJERPH, MDPI, vol. 17(21), pages 1-22, October.
    2. Keerthana Rajagopal & Sujith J. Chandy & Jay P. Graham, 2021. "A One Health Review of Community-Acquired Antimicrobial-Resistant Escherichia coli in India," IJERPH, MDPI, vol. 18(22), pages 1-15, November.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jijerp:v:15:y:2018:i:6:p:1247-:d:152080. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.