IDEAS home Printed from https://ideas.repec.org/a/gam/jijerp/v15y2018i5p1037-d148273.html
   My bibliography  Save this article

Clinical Efficacy Associated with Enhanced Antioxidant Enzyme Activities of Silver Nanoparticles Biosynthesized Using Moringa oleifera Leaf Extract, Against Cutaneous Leishmaniasis in a Murine Model of Leishmania major

Author

Listed:
  • Manal El-khadragy

    (Chair Vaccines Research of Infectious Diseases, Faculty of Science, King Saud University, Riyadh 11451, Saudi Arabia
    Faculty of Science, Zoology Department, King Saud University, Riyadh 11451, Saudi Arabia
    Department of Zoology and Entomology, Faculty of Science, Helwan University, Cairo 11795, Egypt)

  • Ebtesam M. Alolayan

    (Chair Vaccines Research of Infectious Diseases, Faculty of Science, King Saud University, Riyadh 11451, Saudi Arabia
    Faculty of Science, Zoology Department, King Saud University, Riyadh 11451, Saudi Arabia)

  • Dina M. Metwally

    (Chair Vaccines Research of Infectious Diseases, Faculty of Science, King Saud University, Riyadh 11451, Saudi Arabia
    Faculty of Science, Zoology Department, King Saud University, Riyadh 11451, Saudi Arabia
    Parasitology Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt)

  • Mohamed F. Serag El-Din

    (Department of Nutrition and Food Science, Faculty of Home Economics, Minufiya University, Shebin ElKom 32511, Egypt
    Department of Food Sciences, College of Food and Agricultural Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi Arabia)

  • Sara S. Alobud

    (Faculty of Medicine, King Saud University, Riyadh 11451, Saudi Arabia)

  • Nour I. Alsultan

    (Faculty of Medicine, King Saud University, Riyadh 11451, Saudi Arabia)

  • Sarah S. Alsaif

    (Chair Vaccines Research of Infectious Diseases, Faculty of Science, King Saud University, Riyadh 11451, Saudi Arabia
    Faculty of Science, Botany and Microbiology Department, King Saud University, Riyadh 11451, Saudi Arabia)

  • Manal A. Awad

    (King Abdullah Institute for Nanotechnology, King Saud University, Riyadh 11451, Saudi Arabia)

  • Ahmed E. Abdel Moneim

    (Department of Zoology and Entomology, Faculty of Science, Helwan University, Cairo 11795, Egypt)

Abstract

Leishmaniasis is one of the most significant vector-borne syndromes of individuals. This parasitic infection can be affected by many species of Leishmania, most of which are zoonotic. Natural products have made and are continuing to make important contributions to the search for new antileishmanial agents. The use of plants in the production assembly of silver nanoparticles has drawn attention because of its rapid, eco-friendly, non-pathogenic, economical protocol and provides a single step technique for the biosynthetic process. Hence, we aimed to biosynthesize silver nanoparticles (Ag-NPs) using Moringa oleifera leaf extract and investigated the antileishmanial activity of these nanoparticles in a murine model of Leishmania major infection. A total of 50 mice were used and divided into five groups—healthy control, infected, infected mice treated with pentostam, infected mice treated with Ag-NPs and infected mice pretreated with Ag-NPs. In the present study, the leaf extract of the plant species Moringa oleifera was found to be a good source for the synthesis of silver nanoparticles, their formation being confirmed by color change and stability in solution. In the present murine model of Leishmania major infection, we found that oral treatment with silver nanoparticles biosynthesized using Moringa oleifera extract resulted in a significant reduction in the average size of leishmaniasis cutaneous lesions compared with untreated mice. Furthermore, the clinical efficacy of Moringa oleifera extract was associated with enhanced antioxidant enzyme activities. In conclusion, treatment with silver nanoparticles biosynthesized using Moringa oleifera extract has higher and faster clinical efficacy than standard pentavalent antimonial treatment, probably by boosting the endogenous antioxidant activity.

Suggested Citation

  • Manal El-khadragy & Ebtesam M. Alolayan & Dina M. Metwally & Mohamed F. Serag El-Din & Sara S. Alobud & Nour I. Alsultan & Sarah S. Alsaif & Manal A. Awad & Ahmed E. Abdel Moneim, 2018. "Clinical Efficacy Associated with Enhanced Antioxidant Enzyme Activities of Silver Nanoparticles Biosynthesized Using Moringa oleifera Leaf Extract, Against Cutaneous Leishmaniasis in a Murine Model o," IJERPH, MDPI, vol. 15(5), pages 1-14, May.
  • Handle: RePEc:gam:jijerp:v:15:y:2018:i:5:p:1037-:d:148273
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/1660-4601/15/5/1037/pdf
    Download Restriction: no

    File URL: https://www.mdpi.com/1660-4601/15/5/1037/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Maria J. Ribeiro & Vera L. Maria & Janeck J. Scott-Fordsmand & Mónica J. B. Amorim, 2015. "Oxidative Stress Mechanisms Caused by Ag Nanoparticles (NM300K) are Different from Those of AgNO 3 : Effects in the Soil Invertebrate Enchytraeus Crypticus," IJERPH, MDPI, vol. 12(8), pages 1-14, August.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Janeck J. Scott‐Fordsmand & Willie J. G. M. Peijnenburg & Elena Semenzin & Bernd Nowack & Neil Hunt & Danail Hristozov & Antonio Marcomini & Muhammad‐Adeel Irfan & Araceli Sánchez Jiménez & Robert Lan, 2017. "Environmental Risk Assessment Strategy for Nanomaterials," IJERPH, MDPI, vol. 14(10), pages 1-20, October.
    2. Mónica J. B. Amorim, 2016. "The Daunting Challenge of Ensuring Sustainable Development of Nanomaterials," IJERPH, MDPI, vol. 13(2), pages 1-3, February.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jijerp:v:15:y:2018:i:5:p:1037-:d:148273. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.