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Prevalence of Integrons and Insertion Sequences in ESBL-Producing E. coli Isolated from Different Sources in Navarra, Spain

Author

Listed:
  • Lara Pérez-Etayo

    (Department of Microbiology and Parasitology, University of Navarra, 31008 Pamplona, Spain)

  • Melibea Berzosa

    (Department of Microbiology and Parasitology, University of Navarra, 31008 Pamplona, Spain)

  • David González

    (Department of Microbiology and Parasitology, University of Navarra, 31008 Pamplona, Spain
    IDISNA, Navarra Health Research Institute, 31008 Pamplona, Spain)

  • Ana Isabel Vitas

    (Department of Microbiology and Parasitology, University of Navarra, 31008 Pamplona, Spain
    IDISNA, Navarra Health Research Institute, 31008 Pamplona, Spain)

Abstract

Mobile genetic elements play an important role in the dissemination of antibiotic resistant bacteria among human and environmental sources. Therefore, the aim of this study was to determine the occurrence and patterns of integrons and insertion sequences of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli isolated from different sources in Navarra, northern Spain. A total of 150 isolates coming from food products, farms and feeds, aquatic environments, and humans (healthy people and hospital inpatients), were analyzed. PCRs were applied for the study of class 1, 2, and 3 integrons ( intI1 , intI2 , and intI3 ), as well as for the determination of insertion sequences (IS 26 , IS Ecp1 , IS CR1 , and IS 903 ). Results show the wide presence and dissemination of intI1 (92%), while intI3 was not detected. It is remarkable, the prevalence of intI2 among food isolates, as well as the co-existence of class 1 and class 2 (8% of isolates). The majority of isolates have two or three IS elements, with the most common being IS 26 (99.4%). The genetic pattern IS 26 –IS Ecp1 (related with the pathogen clone ST131) was present in the 22% of isolates (including human isolates). In addition, the combination IS Ecp1 –IS 26 –IS 903 –IS CR1 was detected in 11 isolates being, to our knowledge, the first study that describes this genetic complex. Due to the wide variability observed, no relationship was determined among these mobile genetic elements and β-lactam resistance. More investigations regarding the genetic composition of these elements are needed to understand the role of multiple types of integrons and insertion sequences on the dissemination of antimicrobial resistance genes among different environments.

Suggested Citation

  • Lara Pérez-Etayo & Melibea Berzosa & David González & Ana Isabel Vitas, 2018. "Prevalence of Integrons and Insertion Sequences in ESBL-Producing E. coli Isolated from Different Sources in Navarra, Spain," IJERPH, MDPI, vol. 15(10), pages 1-12, October.
    • Handle: RePEc:gam:jijerp:v:15:y:2018:i:10:p:2308-:d:177058
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    References listed on IDEAS

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    1. Elena Ojer-Usoz & David González & Ana Isabel Vitas, 2017. "Clonal Diversity of ESBL-Producing Escherichia coli Isolated from Environmental, Human and Food Samples," IJERPH, MDPI, vol. 14(7), pages 1-13, June.
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