Testis-Specific Lactate Dehydrogenase (LDH-C 4 ) in Skeletal Muscle Enhances Apika’s Sprint-Running Capacity in Hypoxic Environment

LDH-C 4 is a lactate dehydrogenase that catalyzes the conversion of pyruvate to lactate. In mammals, ldh-c was originally thought to be expressed only in testis and spermatozoa. Plateau pika ( Ochotona curzoniae ), which belongs to the genus Ochotona of the Ochotonidea family, is a hypoxia tolerant mammal living 3000–5000 m above sea level on the Qinghai-Tibet Plateau, an environment which is strongly hypoxic. Ldh-c is expressed not only in testis and sperm but also in somatic tissues of plateau pika. In this study, the effects of N -propyl oxamate and N -isopropyl oxamate on LDH isozyme kinetics were compared to screens for a selective inhibitor of LDH-C 4 . To reveal the role and physiological mechanism of LDH-C 4 in skeletal muscle of plateau pika, we investigated the effect of N -isopropyl oxamate on the pika exercise tolerance as well as the physiological mechanism. Our results show that Ki of N -propyl oxamate and N -isopropyl oxamate for LDH-A 4 , LDH-B 4 , and LDH-C 4 were 0.094 mmol/L and 0.462 mmol/L, 0.119 mmol/L and 0.248 mmol/L, and 0.015 mmol/L and 0.013 mmol/L, respectively. N -isopropyl oxamate is a powerful selective inhibitor of plateau pika LDH-C 4 . In our exercise tolerance experiment, groups treated with inhibitors had significantly lower swimming times than the uninhibited control group. The inhibition rates of LDH, LD, and ATP were 37.12%, 66.27%, and 32.42%, respectively. Our results suggested that ldh-c is expressed in the skeletal muscle of plateau pika, and at least 32.42% of ATP in the skeletal muscle is catalyzed by LDH-C 4 by anaerobic glycolysis. This suggests that pika has reduced dependence on oxygen and enhanced adaptation to hypoxic environment due to increased anaerobic glycolysis by LDH-C 4 in skeletal muscle. LDH-C 4 in plateau pika plays the crucial role in anaerobic glycolysis and generates ATP rapidly since this is the role of LDH-A 4 in most species on plain land, which provide evidence that the native humans and animals in Qinghai-Tibet plateau can adapt to the hypoxia environment.