IDEAS home Printed from https://ideas.repec.org/a/gam/jijerp/v11y2014i3p3233-3255d34179.html
   My bibliography  Save this article

Effect of Ampicillin, Streptomycin, Penicillin and Tetracycline on Metal Resistant and Non-Resistant Staphylococcus aureus

Author

Listed:
  • Dagmar Chudobova

    (Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, Brno CZ-613 00, Czech Republic)

  • Simona Dostalova

    (Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, Brno CZ-613 00, Czech Republic)

  • Iva Blazkova

    (Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, Brno CZ-613 00, Czech Republic)

  • Petr Michalek

    (Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, Brno CZ-613 00, Czech Republic)

  • Branislav Ruttkay-Nedecky

    (Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, Brno CZ-613 00, Czech Republic
    Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, Brno CZ-616 00, Czech Republic)

  • Matej Sklenar

    (Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, Brno CZ-613 00, Czech Republic)

  • Lukas Nejdl

    (Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, Brno CZ-613 00, Czech Republic)

  • Jiri Kudr

    (Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, Brno CZ-613 00, Czech Republic)

  • Jaromir Gumulec

    (Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, Brno CZ-616 00, Czech Republic
    Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Komenskeho namesti 2, Brno CZ-662 43, Czech Republic)

  • Katerina Tmejova

    (Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, Brno CZ-613 00, Czech Republic
    Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, Brno CZ-616 00, Czech Republic)

  • Marie Konecna

    (Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, Brno CZ-613 00, Czech Republic
    Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, Brno CZ-616 00, Czech Republic)

  • Marketa Vaculovicova

    (Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, Brno CZ-613 00, Czech Republic
    Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, Brno CZ-616 00, Czech Republic)

  • David Hynek

    (Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, Brno CZ-616 00, Czech Republic)

  • Michal Masarik

    (Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, Brno CZ-616 00, Czech Republic
    Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Komenskeho namesti 2, Brno CZ-662 43, Czech Republic)

  • Jindrich Kynicky

    (Karel Englis College, Sujanovo nam. 356/1, Brno CZ-602 00, Czech Republic)

  • Rene Kizek

    (Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, Brno CZ-613 00, Czech Republic
    Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, Brno CZ-616 00, Czech Republic)

  • Vojtech Adam

    (Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, Brno CZ-613 00, Czech Republic
    Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, Brno CZ-616 00, Czech Republic)

Abstract

There is an arising and concerning issue in the field of bacterial resistance, which is confirmed by the number of deaths associated with drug-resistant bacterial infections. The aim of this study was to compare the effects of antibiotics on Staphylococcus aureus non-resistant strain and strains resistant to cadmium or lead ions. Metal resistant strains were created by the gradual addition of 2 mM solution of metal ions (cadmium or lead) to the S. aureus culture. An increasing antimicrobial effect of ampicillin, streptomycin, penicillin and tetracycline (0, 10, 25, 50, 75, 150, 225 and 300 µM) on the resistant strains was observed using a method of growth curves. A significant growth inhibition (compared to control) of cadmium resistant cells was observed in the presence of all the four different antibiotics. On the other hand, the addition of streptomycin and ampicillin did not inhibit the growth of lead resistant strain. Other antibiotics were still toxic to the bacterial cells. Significant differences in the morphology of cell walls were indicated by changes in the cell shape. Our data show that the presence of metal ions in the urban environment may contribute to the development of bacterial strain resistance to other substances including antibiotics, which would have an impact on public health.

Suggested Citation

  • Dagmar Chudobova & Simona Dostalova & Iva Blazkova & Petr Michalek & Branislav Ruttkay-Nedecky & Matej Sklenar & Lukas Nejdl & Jiri Kudr & Jaromir Gumulec & Katerina Tmejova & Marie Konecna & Marketa , 2014. "Effect of Ampicillin, Streptomycin, Penicillin and Tetracycline on Metal Resistant and Non-Resistant Staphylococcus aureus," IJERPH, MDPI, vol. 11(3), pages 1-23, March.
    • Handle: RePEc:gam:jijerp:v:11:y:2014:i:3:p:3233-3255:d:34179
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/1660-4601/11/3/3233/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/1660-4601/11/3/3233/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Feng Zhou & Yuyan Wang, 2013. "Characteristics of Antibiotic Resistance of Airborne Staphylococcus Isolated from Metro Stations," IJERPH, MDPI, vol. 10(6), pages 1-15, June.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Ewelina Kruszewska & Piotr Czupryna & Sławomir Pancewicz & Diana Martonik & Anna Bukłaha & Anna Moniuszko-Malinowska, 2022. "Is Peracetic Acid Fumigation Effective in Public Transportation?," IJERPH, MDPI, vol. 19(5), pages 1-9, February.
    2. Yueming Wen & Jiawei Leng & Xiaobing Shen & Gang Han & Lijun Sun & Fei Yu, 2020. "Environmental and Health Effects of Ventilation in Subway Stations: A Literature Review," IJERPH, MDPI, vol. 17(3), pages 1-37, February.
    3. Sławomir Kurpaska & Katarzyna Wolny-Koładka & Mateusz Malinowski & Klaudia Tomaszek & Hubert Latała, 2023. "Thermal-Mass and Microbiological Analysis of Forced Air Flow through the Stone Heat Accumulator Bed," Energies, MDPI, vol. 16(11), pages 1-22, May.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jijerp:v:11:y:2014:i:3:p:3233-3255:d:34179. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.