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Optimal Method to Stimulate Cytokine Production and Its Use in Immunotoxicity Assessment

Author

Listed:
  • Wenchao Ai

    (Chinese Academy of Inspection and Quarantine, Beijing 100123, China)

  • Haishan Li

    (Chinese Academy of Inspection and Quarantine, Beijing 100123, China)

  • Naining Song

    (Chinese Academy of Inspection and Quarantine, Beijing 100123, China)

  • Lei Li

    (Chinese Academy of Inspection and Quarantine, Beijing 100123, China)

  • Huiming Chen

    (Chinese Academy of Inspection and Quarantine, Beijing 100123, China)

Abstract

Activation of lymphocytes can effectively produce a large amount of cytokines. The types of cytokines produced may depend on stimulating reagents and treatments. To find an optimal method to stimulate cytokine production and evaluate its effect on immunotoxicity assessments, the authors analyzed production of IL-2, IL-4, IL-6, IL-10, IL-13, IFN-γ, TNF-α, GM-CSF, RANTES and TGF-β in undiluted rat whole blood culture (incubation for 0, 2, 4, 6, 8 or 10 h) with different concentrations of PMA/ionomycin, PHA, Con A, LPS and PWM. We also evaluated the effects of cyclosporin A and azathioprine on cytokine production. The results revealed a rapid increase of IL-2, IFN-γ, TNF-α, RANTES and TGF-β secretion within 6 h after stimulation with 25 ng/mL PMA and 1 μg/mL ionomycin. The inhibition of these cytokine profiles reflected the effects of immunosuppressants on the immune system. Therefore, the results of this is study recommend the detection of cytokine profiles in undiluted whole blood stimulated 6 h with 25 ng/mL PMA and 1 μg/mL ionomycin as a powerful immunotoxicity assessment method.

Suggested Citation

  • Wenchao Ai & Haishan Li & Naining Song & Lei Li & Huiming Chen, 2013. "Optimal Method to Stimulate Cytokine Production and Its Use in Immunotoxicity Assessment," IJERPH, MDPI, vol. 10(9), pages 1-9, August.
    • Handle: RePEc:gam:jijerp:v:10:y:2013:i:9:p:3834-3842:d:28304
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