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Detection of β-Lactamase Residues in Milk by Sandwich ELISA

Author

Listed:
  • Wenbin Wang

    (State Key Laboratory of Food Science & Technology, School of Food Science & Technology, Jiangnan University, Wuxi 214122, China)

  • Liqiang Liu

    (State Key Laboratory of Food Science & Technology, School of Food Science & Technology, Jiangnan University, Wuxi 214122, China)

  • Liguang Xu

    (State Key Laboratory of Food Science & Technology, School of Food Science & Technology, Jiangnan University, Wuxi 214122, China)

  • Wei Ma

    (State Key Laboratory of Food Science & Technology, School of Food Science & Technology, Jiangnan University, Wuxi 214122, China)

  • Hua Kuang

    (State Key Laboratory of Food Science & Technology, School of Food Science & Technology, Jiangnan University, Wuxi 214122, China)

  • Chuanlai Xu

    (State Key Laboratory of Food Science & Technology, School of Food Science & Technology, Jiangnan University, Wuxi 214122, China)

Abstract

β-Lactamase residues in milk represent a public health risk. The cylinder plate detection method, which is based on bacterial growth, is laborious and time consuming. In this study, 15 monoclonal antibodies (mAbs) were selected against Temoneira (TEM) 1 β-lactamase. A sandwich enzyme-linked immunosorbent assay (ELISA) based on an optimum mAb pair was developed and validated for the detection of β-lactamase. The limit of detection and linear dynamic range of the method were 4.17 ng/mL and 5.5–100 ng/mL, respectively. β-Lactamase recovery in pure milk was 96.82–103.13%. The intra- and inter-assay coefficients of variation were 6.21–7.38% and 12.96–13.74%, respectively. Our developed sandwich ELISA can be used as a rapid detection method of β-lactamase in milk.

Suggested Citation

  • Wenbin Wang & Liqiang Liu & Liguang Xu & Wei Ma & Hua Kuang & Chuanlai Xu, 2013. "Detection of β-Lactamase Residues in Milk by Sandwich ELISA," IJERPH, MDPI, vol. 10(7), pages 1-11, June.
    • Handle: RePEc:gam:jijerp:v:10:y:2013:i:7:p:2688-2698:d:26799
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    References listed on IDEAS

    as
    1. Hua Kuang & Wenbing Wang & Liguang Xu & Wei Ma & Liqiang Liu & Libing Wang & Chuanlai Xu, 2013. "Monoclonal Antibody-Based Sandwich ELISA for the Detection of Staphylococcal Enterotoxin A," IJERPH, MDPI, vol. 10(4), pages 1-11, April.
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