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Monoclonal Antibody-Based Sandwich ELISA for the Detection of Staphylococcal Enterotoxin A

Author

Listed:
  • Hua Kuang

    (State Key Laboratory of Food Science & Technology, School of Food Science & Technology, Jiangnan University, Wuxi 214122, China
    These authors contributed equally to this work.)

  • Wenbing Wang

    (State Key Laboratory of Food Science & Technology, School of Food Science & Technology, Jiangnan University, Wuxi 214122, China
    These authors contributed equally to this work.)

  • Liguang Xu

    (State Key Laboratory of Food Science & Technology, School of Food Science & Technology, Jiangnan University, Wuxi 214122, China)

  • Wei Ma

    (State Key Laboratory of Food Science & Technology, School of Food Science & Technology, Jiangnan University, Wuxi 214122, China)

  • Liqiang Liu

    (State Key Laboratory of Food Science & Technology, School of Food Science & Technology, Jiangnan University, Wuxi 214122, China)

  • Libing Wang

    (Research Centre of Hunan Entry-Exit Inspection and Quarantine Bureau, Changsha 410001, China)

  • Chuanlai Xu

    (State Key Laboratory of Food Science & Technology, School of Food Science & Technology, Jiangnan University, Wuxi 214122, China)

Abstract

A sensitive and specific monoclonal antibody-based sandwich enzyme-linked immunosorbent assay (ELISA) was established and validated for the detection of staphylococcal enterotoxin A (SEA). After routine fusion and selection, 10 monoclonal antibodies showed high affinity for SEA. An optimal pair for sandwich ELISA was selected by pairwise interaction analysis. After optimization, the limit of detection (LOD) and linear dynamic range of the method were established, and were found to be 0.0282 ng/mL and 0.06–2 ng/mL, respectively. The recovery in pure milk ranged from 82.67% to 111.95% and the intra- and inter-assay coefficients of variation ranged from 3.16% to 6.05% and from 5.16% to 10.79%, respectively. Cross-reactivity with staphylococcal enterotoxin B (SEB), staphylococcal enterotoxin C (SEC), staphylococcal enterotoxin D (SED), and staphylococcal enterotoxin E (SEE) in this method were insignificant. These results indicate that the sandwich ELISA method developed in our study is effective for routine identification of SEA in food samples.

Suggested Citation

  • Hua Kuang & Wenbing Wang & Liguang Xu & Wei Ma & Liqiang Liu & Libing Wang & Chuanlai Xu, 2013. "Monoclonal Antibody-Based Sandwich ELISA for the Detection of Staphylococcal Enterotoxin A," IJERPH, MDPI, vol. 10(4), pages 1-11, April.
    • Handle: RePEc:gam:jijerp:v:10:y:2013:i:4:p:1598-1608:d:25138
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    Cited by:

    1. Wenbin Wang & Liqiang Liu & Liguang Xu & Wei Ma & Hua Kuang & Chuanlai Xu, 2013. "Detection of β-Lactamase Residues in Milk by Sandwich ELISA," IJERPH, MDPI, vol. 10(7), pages 1-11, June.

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