IDEAS home Printed from https://ideas.repec.org/a/gam/jeners/v15y2022i6p2182-d772887.html
   My bibliography  Save this article

Immobilizing Enzymes on a Commercial Polymer: Performance Analysis of a GOx-Laccase Based Enzymatic Biofuel Cell Assembly

Author

Listed:
  • Dario Pelosi

    (Department of Engineering, University of Perugia, Via G. Duranti 93, 06125 Perugia, Italy)

  • Linda Barelli

    (Department of Engineering, University of Perugia, Via G. Duranti 93, 06125 Perugia, Italy)

  • Nicolò Montegiove

    (Department of Chemistry, Biology and Biotechnology, University of Perugia, Via del Giochetto, 06123 Perugia, Italy)

  • Eleonora Calzoni

    (Department of Chemistry, Biology and Biotechnology, University of Perugia, Via del Giochetto, 06123 Perugia, Italy)

  • Alessio Cesaretti

    (Department of Chemistry, Biology and Biotechnology, University of Perugia, Via del Giochetto, 06123 Perugia, Italy
    Centro di Eccellenza sui Materiali Innovativi Nanostrutturati (CEMIN), University of Perugia, Via Elce di Sotto 8, 06123 Perugia, Italy)

  • Alessandro Di Michele

    (Department of Physics and Geology, University of Perugia, Via Pascoli, 06123 Perugia, Italy)

  • Carla Emiliani

    (Department of Chemistry, Biology and Biotechnology, University of Perugia, Via del Giochetto, 06123 Perugia, Italy
    Centro di Eccellenza sui Materiali Innovativi Nanostrutturati (CEMIN), University of Perugia, Via Elce di Sotto 8, 06123 Perugia, Italy)

  • Luca Gammaitoni

    (Department of Physics and Geology, University of Perugia, Via Pascoli, 06123 Perugia, Italy)

Abstract

Enzymatic Biofuel Cell (EBC) represents a promising green source since it is capable of harvesting electricity from renewable and abundantly available biofuels using enzymes as catalysts. Nevertheless, nowadays long-term stability and low power output are currently the main concerns. To this end, several research studies focus on using complex tridimensional and highly expensive nanostructures as electrode support for enzymes. This increases cell performance whilst drastically reducing the economic feasibility needed for industrial viability. Thus, this paper analyzes a novel flow-based EBC consisting of covalent immobilized GOx (bioanode) and Laccase (biocathode) on a commercial flat conductive polymer. A suitable immobilization technique based on covalent ligands is carried out to enhance EBC durability. The experimental characterization demonstrates that the cell generates power over three weeks, reaching 590 mV and 2.41 µW cm −2 as maximum open circuit voltage and power density, respectively. The most significant contributions of this configuration are definitely ease of implementation, low cost, high scalability, and reproducibility. Therefore, such a design can be considered a step forward in the viable EBC industrialization process for a wide range of applications.

Suggested Citation

  • Dario Pelosi & Linda Barelli & Nicolò Montegiove & Eleonora Calzoni & Alessio Cesaretti & Alessandro Di Michele & Carla Emiliani & Luca Gammaitoni, 2022. "Immobilizing Enzymes on a Commercial Polymer: Performance Analysis of a GOx-Laccase Based Enzymatic Biofuel Cell Assembly," Energies, MDPI, vol. 15(6), pages 1-12, March.
    • Handle: RePEc:gam:jeners:v:15:y:2022:i:6:p:2182-:d:772887
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/1996-1073/15/6/2182/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/1996-1073/15/6/2182/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Violetta Vasilenko & Irina Arkadeva & Vera Bogdanovskaya & George Sudarev & Sergei Kalenov & Marco Vocciante & Eleonora Koltsova, 2020. "Glucose-Oxygen Biofuel Cell with Biotic and Abiotic Catalysts: Experimental Research and Mathematical Modeling," Energies, MDPI, vol. 13(21), pages 1-21, October.
    2. Linda Barelli & Gianni Bidini & Dario Pelosi & Elena Sisani, 2021. "Enzymatic Biofuel Cells: A Review on Flow Designs," Energies, MDPI, vol. 14(4), pages 1-26, February.
    3. Ivan Ivanov & Tanja Vidaković-Koch & Kai Sundmacher, 2010. "Recent Advances in Enzymatic Fuel Cells: Experiments and Modeling," Energies, MDPI, vol. 3(4), pages 1-44, April.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Attilio Converti, 2021. "Environmental and Energetic Valorization of Renewable Resources," Energies, MDPI, vol. 14(24), pages 1-5, December.
    2. Kim, Seongjun & Ji, Jungyeon & Kwon, Yongchai, 2023. "Paper-type membraneless enzymatic biofuel cells using a new biocathode consisting of flexible buckypaper electrode and bilirubin oxidase based catalyst modified by electrografting," Applied Energy, Elsevier, vol. 339(C).
    3. Linda Barelli & Gianni Bidini & Dario Pelosi & Elena Sisani, 2021. "Enzymatic Biofuel Cells: A Review on Flow Designs," Energies, MDPI, vol. 14(4), pages 1-26, February.
    4. Violetta Vasilenko & Irina Arkadeva & Vera Bogdanovskaya & George Sudarev & Sergei Kalenov & Marco Vocciante & Eleonora Koltsova, 2020. "Glucose-Oxygen Biofuel Cell with Biotic and Abiotic Catalysts: Experimental Research and Mathematical Modeling," Energies, MDPI, vol. 13(21), pages 1-21, October.
    5. Ihor Sobianin & Sotiria D. Psoma & Antonios Tourlidakis, 2022. "Recent Advances in Energy Harvesting from the Human Body for Biomedical Applications," Energies, MDPI, vol. 15(21), pages 1-24, October.
    6. Der-Sheng Chan & Der-Jong Dai & Ho-Shing Wu, 2012. "Dynamic Modeling of Anode Function in Enzyme-Based Biofuel Cells Using High Mediator Concentration," Energies, MDPI, vol. 5(7), pages 1-21, July.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jeners:v:15:y:2022:i:6:p:2182-:d:772887. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.