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Immobilizing Enzymes on a Commercial Polymer: Performance Analysis of a GOx-Laccase Based Enzymatic Biofuel Cell Assembly

Author

Listed:
  • Dario Pelosi

    (Department of Engineering, University of Perugia, Via G. Duranti 93, 06125 Perugia, Italy)

  • Linda Barelli

    (Department of Engineering, University of Perugia, Via G. Duranti 93, 06125 Perugia, Italy)

  • Nicolò Montegiove

    (Department of Chemistry, Biology and Biotechnology, University of Perugia, Via del Giochetto, 06123 Perugia, Italy)

  • Eleonora Calzoni

    (Department of Chemistry, Biology and Biotechnology, University of Perugia, Via del Giochetto, 06123 Perugia, Italy)

  • Alessio Cesaretti

    (Department of Chemistry, Biology and Biotechnology, University of Perugia, Via del Giochetto, 06123 Perugia, Italy
    Centro di Eccellenza sui Materiali Innovativi Nanostrutturati (CEMIN), University of Perugia, Via Elce di Sotto 8, 06123 Perugia, Italy)

  • Alessandro Di Michele

    (Department of Physics and Geology, University of Perugia, Via Pascoli, 06123 Perugia, Italy)

  • Carla Emiliani

    (Department of Chemistry, Biology and Biotechnology, University of Perugia, Via del Giochetto, 06123 Perugia, Italy
    Centro di Eccellenza sui Materiali Innovativi Nanostrutturati (CEMIN), University of Perugia, Via Elce di Sotto 8, 06123 Perugia, Italy)

  • Luca Gammaitoni

    (Department of Physics and Geology, University of Perugia, Via Pascoli, 06123 Perugia, Italy)

Abstract

Enzymatic Biofuel Cell (EBC) represents a promising green source since it is capable of harvesting electricity from renewable and abundantly available biofuels using enzymes as catalysts. Nevertheless, nowadays long-term stability and low power output are currently the main concerns. To this end, several research studies focus on using complex tridimensional and highly expensive nanostructures as electrode support for enzymes. This increases cell performance whilst drastically reducing the economic feasibility needed for industrial viability. Thus, this paper analyzes a novel flow-based EBC consisting of covalent immobilized GOx (bioanode) and Laccase (biocathode) on a commercial flat conductive polymer. A suitable immobilization technique based on covalent ligands is carried out to enhance EBC durability. The experimental characterization demonstrates that the cell generates power over three weeks, reaching 590 mV and 2.41 µW cm −2 as maximum open circuit voltage and power density, respectively. The most significant contributions of this configuration are definitely ease of implementation, low cost, high scalability, and reproducibility. Therefore, such a design can be considered a step forward in the viable EBC industrialization process for a wide range of applications.

Suggested Citation

  • Dario Pelosi & Linda Barelli & Nicolò Montegiove & Eleonora Calzoni & Alessio Cesaretti & Alessandro Di Michele & Carla Emiliani & Luca Gammaitoni, 2022. "Immobilizing Enzymes on a Commercial Polymer: Performance Analysis of a GOx-Laccase Based Enzymatic Biofuel Cell Assembly," Energies, MDPI, vol. 15(6), pages 1-12, March.
    • Handle: RePEc:gam:jeners:v:15:y:2022:i:6:p:2182-:d:772887
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    References listed on IDEAS

    as
    1. Linda Barelli & Gianni Bidini & Dario Pelosi & Elena Sisani, 2021. "Enzymatic Biofuel Cells: A Review on Flow Designs," Energies, MDPI, vol. 14(4), pages 1-26, February.
    2. Violetta Vasilenko & Irina Arkadeva & Vera Bogdanovskaya & George Sudarev & Sergei Kalenov & Marco Vocciante & Eleonora Koltsova, 2020. "Glucose-Oxygen Biofuel Cell with Biotic and Abiotic Catalysts: Experimental Research and Mathematical Modeling," Energies, MDPI, vol. 13(21), pages 1-21, October.
    3. Ivan Ivanov & Tanja Vidaković-Koch & Kai Sundmacher, 2010. "Recent Advances in Enzymatic Fuel Cells: Experiments and Modeling," Energies, MDPI, vol. 3(4), pages 1-44, April.
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