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Metagenomic Analysis of the Long-Term Synergistic Effects of Antibiotics on the Anaerobic Digestion of Cattle Manure

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Listed:
  • Izabela Wolak

    (Department of Water Protection Engineering and Environmental Microbiology, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 1, 10-720 Olsztyn, Poland)

  • Małgorzata Czatzkowska

    (Department of Water Protection Engineering and Environmental Microbiology, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 1, 10-720 Olsztyn, Poland)

  • Monika Harnisz

    (Department of Water Protection Engineering and Environmental Microbiology, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 1, 10-720 Olsztyn, Poland)

  • Jan Paweł Jastrzębski

    (Department of Physiology, Genetics and Plant Biotechnology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-957 Olsztyn, Poland)

  • Łukasz Paukszto

    (Department of Botany and Nature Protection, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Plac Łódzki 1, 10-721 Olsztyn, Poland)

  • Paulina Rusanowska

    (Department of Environmental Engineering, University of Warmia and Mazury in Olsztyn, Warszawska 117, 10-950 Olsztyn, Poland)

  • Ewa Felis

    (Department of Environmental Biotechnology, Faculty of Energy and Environmental Engineering, Silesian University of Technology, Akademicka 2, 44-100 Gliwice, Poland)

  • Ewa Korzeniewska

    (Department of Water Protection Engineering and Environmental Microbiology, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 1, 10-720 Olsztyn, Poland)

Abstract

The conversion of cattle manure into biogas in anaerobic digestion (AD) processes has been gaining attention in recent years. However, antibiotic consumption continues to increase worldwide, which is why antimicrobial concentrations can be expected to rise in cattle manure and in digestate. This study examined the long-term synergistic effects of antimicrobials on the anaerobic digestion of cattle manure. The prevalence of antibiotic resistance genes (ARGs) and changes in microbial biodiversity under exposure to the tested drugs was investigated using a metagenomic approach. Methane production was analyzed in lab-scale anaerobic bioreactors. Bacteroidetes , Firmicutes, and Actinobacteria were the most abundant bacteria in the samples. The domain Archaea was represented mainly by methanogenic genera Methanothrix and Methanosarcina and the order Methanomassiliicoccales . Exposure to antibiotics inhibited the growth and development of methanogenic microorganisms in the substrate. Antibiotics also influenced the abundance and prevalence of ARGs in samples. Seventeen types of ARGs were identified and classified. Genes encoding resistance to tetracyclines, macrolide–lincosamide–streptogramin antibiotics, and aminoglycosides, as well as multi-drug resistance genes, were most abundant. Antibiotics affected homoacetogenic bacteria and methanogens, and decreased the production of CH 4 . However, the antibiotic-induced decrease in CH 4 production was minimized in the presence of highly drug-resistant microorganisms in AD bioreactors.

Suggested Citation

  • Izabela Wolak & Małgorzata Czatzkowska & Monika Harnisz & Jan Paweł Jastrzębski & Łukasz Paukszto & Paulina Rusanowska & Ewa Felis & Ewa Korzeniewska, 2022. "Metagenomic Analysis of the Long-Term Synergistic Effects of Antibiotics on the Anaerobic Digestion of Cattle Manure," Energies, MDPI, vol. 15(5), pages 1-19, March.
    • Handle: RePEc:gam:jeners:v:15:y:2022:i:5:p:1920-:d:765310
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    References listed on IDEAS

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    1. Izabela Koniuszewska & Monika Harnisz & Ewa Korzeniewska & Małgorzata Czatzkowska & Jan Paweł Jastrzębski & Łukasz Paukszto & Sylwia Bajkacz & Ewa Felis & Paulina Rusanowska, 2021. "The Effect of Antibiotics on Mesophilic Anaerobic Digestion Process of Cattle Manure," Energies, MDPI, vol. 14(4), pages 1-19, February.
    2. Chris S. Smillie & Mark B. Smith & Jonathan Friedman & Otto X. Cordero & Lawrence A. David & Eric J. Alm, 2011. "Ecology drives a global network of gene exchange connecting the human microbiome," Nature, Nature, vol. 480(7376), pages 241-244, December.
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