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Adaptive Laboratory Evolution for Multistress Tolerance, including Fermentability at High Glucose Concentrations in Thermotolerant Candida tropicalis

Author

Listed:
  • Koudkeo Phommachan

    (Graduate School of Sciences and Technology for Innovation, Yamaguchi University, Yamaguchi 753-8515, Japan)

  • Chansom Keo-oudone

    (Department of Biology, Faculty of Natural Science, National University of Laos, Vientiane 7322, Laos)

  • Mochamad Nurcholis

    (Department of Food Science and Technology, Faculty of Agricultural Technology, Brawijaya University, Malang 65145, Indonesia)

  • Nookhao Vongvilaisak

    (Department of Biology, Faculty of Natural Science, National University of Laos, Vientiane 7322, Laos)

  • Mingkhuan Chanhming

    (Department of Biology, Faculty of Natural Science, National University of Laos, Vientiane 7322, Laos)

  • Vanhnavong Savanhnaly

    (Department of Biology, Faculty of Natural Science, National University of Laos, Vientiane 7322, Laos)

  • Somchanh Bounphanmy

    (Department of Biology, Faculty of Natural Science, National University of Laos, Vientiane 7322, Laos)

  • Minenosuke Matsutani

    (Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753-8515, Japan
    Present address: Minenosuke Matsutani, NODAI Genome Research Center, Tokyo University of Agriculture, Tokyo 156-8502, Japan.)

  • Tomoyuki Kosaka

    (Graduate School of Sciences and Technology for Innovation, Yamaguchi University, Yamaguchi 753-8515, Japan
    Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753-8515, Japan
    Research Center for Thermotolerant Microbial Resources, Yamaguchi University, Yamaguchi 753-8315, Japan)

  • Savitree Limtong

    (Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand)

  • Mamoru Yamada

    (Graduate School of Sciences and Technology for Innovation, Yamaguchi University, Yamaguchi 753-8515, Japan
    Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753-8515, Japan
    Research Center for Thermotolerant Microbial Resources, Yamaguchi University, Yamaguchi 753-8315, Japan)

Abstract

Candida tropicalis , a xylose-fermenting yeast, has the potential for converting cellulosic biomass to ethanol. Thermotolerant C . tropicalis X-17, which was isolated in Laos, was subjected to repetitive long-term cultivation with a gradual increase in temperature (RLCGT) in the presence of a high concentration of glucose, which exposed cells to various stresses in addition to the high concentration of glucose and high temperatures. The resultant adapted strain demonstrated increased tolerance to ethanol, furfural and hydroxymethylfurfural at high temperatures and displayed improvement in fermentation ability at high glucose concentrations and xylose-fermenting ability. Transcriptome analysis revealed the up-regulation of a gene for a glucose transporter of the major facilitator superfamily and genes for stress response and cell wall proteins. Additionally, hydropathy analysis revealed that three genes for putative membrane proteins with multiple membrane-spanning segments were also up-regulated. From these findings, it can be inferred that the up-regulation of genes, including the gene for a glucose transporter, is responsible for the phenotype of the adaptive strain. This study revealed part of the mechanisms of fermentability at high glucose concentrations in C. tropicalis and the results of this study suggest that RLCGT is an effective procedure for improving multistress tolerance.

Suggested Citation

  • Koudkeo Phommachan & Chansom Keo-oudone & Mochamad Nurcholis & Nookhao Vongvilaisak & Mingkhuan Chanhming & Vanhnavong Savanhnaly & Somchanh Bounphanmy & Minenosuke Matsutani & Tomoyuki Kosaka & Savit, 2022. "Adaptive Laboratory Evolution for Multistress Tolerance, including Fermentability at High Glucose Concentrations in Thermotolerant Candida tropicalis," Energies, MDPI, vol. 15(2), pages 1-15, January.
    • Handle: RePEc:gam:jeners:v:15:y:2022:i:2:p:561-:d:724029
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    References listed on IDEAS

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    1. Hoekman, S. Kent, 2009. "Biofuels in the U.S. – Challenges and Opportunities," Renewable Energy, Elsevier, vol. 34(1), pages 14-22.
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