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Comparative Analyses of Green Plantlet Regeneration in Barley ( Hordeum vulgare L.) Anther Culture

Author

Listed:
  • Csaba Lantos

    (Cereal Research Non-Profit Ltd., P.O. Box 391, H-6701 Szeged, Hungary)

  • Ferenc Markó

    (Cereal Research Non-Profit Ltd., P.O. Box 391, H-6701 Szeged, Hungary)

  • Róbert Mihály

    (Cereal Research Non-Profit Ltd., P.O. Box 391, H-6701 Szeged, Hungary)

  • János Pauk

    (Cereal Research Non-Profit Ltd., P.O. Box 391, H-6701 Szeged, Hungary)

Abstract

The efficient doubled haploid (DH) plant production methods play a key role in accelerating the breeding of new varieties and hybrids in cultivated plants. Consequently, DH plant production methods are continuously improving for barley ( Hordeum vulgare L.) breeding and research programs. Two plant regeneration (FHGR and K4NB) and three rooting media (MSr, N6I and ½N6I + Ca) were compared with four F 1 barley cross-combinations to clarify the effect of medium on the regeneration of green and albino plantlets and acclimatization. The plant regeneration efficiency was higher using K4NB medium (74.53 green plantlets/100 anthers and 30.85 albino/100 anthers) compared to FHGR (55.77 green plantlets/100anthers and 21.32 albino/100 anthers). The percentage of acclimatization was highest when the K4NB regeneration medium was combined with the MSr rooting medium. Altogether, 61.83% of the anther culture-derived plantlets of 8 cross-combinations acclimatized to the greenhouse conditions, and 1403 acclimatized plantlets were produced from the F 1 cross-combinations. Haploid (22.52%), diploid (69.37%) and tetraploid (8.11%) plantlets were identified among the 111 tested green plantlets by flow cytometric analyses. The tetraploid lines can be explored to offer new scopes for future barley research and breeding directions. Nearly one thousand DH plants have been integrated into our barley breeding program.

Suggested Citation

  • Csaba Lantos & Ferenc Markó & Róbert Mihály & János Pauk, 2024. "Comparative Analyses of Green Plantlet Regeneration in Barley ( Hordeum vulgare L.) Anther Culture," Agriculture, MDPI, vol. 14(9), pages 1-13, August.
    • Handle: RePEc:gam:jagris:v:14:y:2024:i:9:p:1440-:d:1463152
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    References listed on IDEAS

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    1. Katarzyna MAKOWSKA & Sylwia OLESZCZUK & Janusz ZIMNY, 2017. "The effect of copper on plant regeneration in barley microspore culture," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 53(1), pages 17-22.
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