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Efficiency of three haplomethods in durum wheat (Triticum turgidum subsp. durum Desf.): isolated microspore culture, gynogenesis and wheat × maize crosses

Author

Listed:
  • Olfa Slama-Ayed

    (Genetic and Cereal Breeding Laboratory, National Agronomic Institute of Tunisia, University of Carthage, Tunis Mahrajene, Tunisia)

  • Imen Bouhaouel

    (Genetic and Cereal Breeding Laboratory, National Agronomic Institute of Tunisia, University of Carthage, Tunis Mahrajene, Tunisia)

  • Sourour Ayed

    (Genetic and Cereal Breeding Laboratory, National Agronomic Institute of Tunisia, University of Carthage, Tunis Mahrajene, Tunisia
    Field Crop Laboratory, Regional Research Development Office of Agriculture in Semi-Arid North West of Kef, Kef, Tunisia)

  • Jacques De Buyser

    (MVEH Laboratory, Paris-Sud University, Orsay, France)

  • Emmanuel Picard

    (MVEH Laboratory, Paris-Sud University, Orsay, France)

  • Hajer Slim Amara

    (Genetic and Cereal Breeding Laboratory, National Agronomic Institute of Tunisia, University of Carthage, Tunis Mahrajene, Tunisia)

Abstract

This study presents the first report comparing the efficiency of microspore culture, gynogenesis and durum wheat × maize crosses for haploid plant production from three durum wheat genotypes (Razzek, Karim and Jneh Khotifa). The results showed that the best induction, calli or embryos formation and plant regeneration rates for the three genotypes were obtained with gynogenesis (47.2, 7.6, 0.8%), followed by interspecific crosses (33.1, 1.7, 0.4%) and isolated microspore culture (8.2, 0.05, 0.01%). Interestingly, all plants regenerated by gynogenesis and durum wheat × maize crosses were green whereas all plants obtained by isolated microspore culture were albino. In the haploid production system, all steps of the process are important for the three methods. The critical steps that have greatly reduced the number of regenerated haploid plants were induction, embryogenesis and regeneration for microspore culture, forming and regeneration of calli or embryo and haploid regeneration for interspecific crosses and gynogenesis. Genotypes with good capacity of induction have not necessarily a good capacity of haploid plantlets regeneration and vice-versa. However, calli or embryos formation seems to be an indicator of the haploid production. Overall, Razzek showed a good ability to produce haploids using the three methods. Each haplomethod showed a specific advantage. Although gynogenesis is the less used method for durum wheat, it has proved to be a successful approach for green haploid plant production.

Suggested Citation

  • Olfa Slama-Ayed & Imen Bouhaouel & Sourour Ayed & Jacques De Buyser & Emmanuel Picard & Hajer Slim Amara, 2019. "Efficiency of three haplomethods in durum wheat (Triticum turgidum subsp. durum Desf.): isolated microspore culture, gynogenesis and wheat × maize crosses," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 55(3), pages 101-109.
    • Handle: RePEc:caa:jnlcjg:v:55:y:2019:i:3:id:188-2017-cjgpb
    DOI: 10.17221/188/2017-CJGPB
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    References listed on IDEAS

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    1. Ugur BAL & Mehran E. SHARIATPANAHI & Antonio J. CASTRO & Delphine EMERY & Christophe CLÉMENT & Maryam DEHESTANI-ARDAKANI & Khadijeh MOZAFFARI & Alisher TOURAEV, 2012. "Pseudo-embryogenic structures in anther and isolated microspore cultures in vitro: a cautionary guide," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 48(2), pages 51-60.
    2. Harinder Kumar CHAUDHARY & Anima MAHATO & Vineeta KAILA & Shoukat Ahmad RATHER & Tisu TAYENG, 2015. "Dihaploid induction in tetraploid durum wheat (Triticum durum L.) using pollen of Imperata cylindrica," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 51(4), pages 142-147.
    3. Katarzyna MAKOWSKA & Sylwia OLESZCZUK & Janusz ZIMNY, 2017. "The effect of copper on plant regeneration in barley microspore culture," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 53(1), pages 17-22.
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