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A Comparative Transcriptomic and Proteomic Analysis of the Pileus of Agaricus bisporus During Its Different Developmental Phases

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  • Weilin Feng

    (Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
    These authors contributed equally to this study.)

  • Zier Guo

    (Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
    College of Biology and Environment, Zhejiang Wanli University, Ningbo 315100, China
    These authors contributed equally to this study.)

  • Qunli Jin

    (Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China)

  • Yingyue Shen

    (Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China)

  • Tingting Song

    (Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China)

  • Mei Wang

    (Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China)

  • Jun Zhang

    (Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China)

  • Lijun Fan

    (Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China)

  • Weiming Cai

    (Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China)

Abstract

The analysis of the developmental stages of Agaricus bisporus , a major edible and medicinal mushroom, has always been an important focus in this research area. The process of the growth and development of edible mushrooms is complex and involves the regulation of multiple genes and metabolic pathways. Less data exist on the mechanism of their growth and development at the overall level. In this study, RNA sequencing analyses (RNA-Seq) and data-independent acquisition (DIA) proteomic analyses were carried out at the button phase (BP), harvesting phase (HP), and opening phase (OP) stages of Agaricus bisporus ‘Shuangbao 106’ to reveal the changes in gene expression during the different growth periods of its substrates. The authors screened and explored 3351 differentially expressed genes (DEGs) with a difference factor of ≥2.0, including 2080 up-regulated and 1271 down-regulated genes. After proteome sequencing, 1156 differentially expressed proteins (DEPs) were screened, including 524 up-regulated and 632 down-regulated genes. The expression in TPM of glycoside hydrolase, catalytic core, and chitinase II decreased during both the HP and OP compared with the BP. This may be because mushrooms require higher levels of glycoside hydrolase, catalytic core, and chitinase II activity during the BP to cope with external threats and the need for cell wall remodeling. Conversely, the growth of mushrooms slowed down and the need for cell wall remodeling decreased during the HP and OP, leading to a decrease in the expression of glycoside hydrolase, catalytic core, and chitinase II. This change is related to the need for environmental adaptation, immune defense, and cell wall remodeling, and may regulate the post-growth process of A. bisporus via the hydrolysis of cell wall chitin and glycoside hydrolase. It may also inhibit the growth of mushroom pilei.

Suggested Citation

  • Weilin Feng & Zier Guo & Qunli Jin & Yingyue Shen & Tingting Song & Mei Wang & Jun Zhang & Lijun Fan & Weiming Cai, 2024. "A Comparative Transcriptomic and Proteomic Analysis of the Pileus of Agaricus bisporus During Its Different Developmental Phases," Agriculture, MDPI, vol. 14(12), pages 1-19, December.
    • Handle: RePEc:gam:jagris:v:14:y:2024:i:12:p:2226-:d:1537383
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    References listed on IDEAS

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    1. François Dragon & Jennifer E. G. Gallagher & Patricia A. Compagnone-Post & Brianna M. Mitchell & Kara A. Porwancher & Karen A. Wehner & Steven Wormsley & Robert E. Settlage & Jeffrey Shabanowitz & Yvo, 2002. "A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesis," Nature, Nature, vol. 417(6892), pages 967-970, June.
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