IDEAS home Printed from https://ideas.repec.org/a/gam/jagris/v13y2023i4p886-d1125730.html
   My bibliography  Save this article

Isolation of Glyphosate-Resistant Bacterial Strains to Improve the Growth of Maize and Degrade Glyphosate under Axenic Condition

Author

Listed:
  • Waqas Mohy-Ud-Din

    (Institute of Soil and Environmental Sciences, University of Agriculture, Faisalabad 38040, Pakistan)

  • Muhammad Javed Akhtar

    (Institute of Soil and Environmental Sciences, University of Agriculture, Faisalabad 38040, Pakistan)

  • Safdar Bashir

    (Department of Soil and Environmental Sciences, Ghazi University, Dera Ghazi Khan 32200, Pakistan)

  • Hafiz Naeem Asghar

    (Institute of Soil and Environmental Sciences, University of Agriculture, Faisalabad 38040, Pakistan)

  • Muhammad Farrakh Nawaz

    (Department of Forestry & Range Management, University of Agriculture, Faisalabad 38040, Pakistan
    Institute of Environmental Studies, University of Karachi, Karachi 75270, Pakistan)

  • Feng Chen

    (Institute of Marine and Environmental Technology, University of Maryland Center for Environmental Science, Baltimore, MD 21202, USA)

Abstract

Glyphosate is a non-selective herbicide that is used to control perennial weeds in agriculture. However, its vast application may result in glyphosate residues in the food chain. Due to its toxicity to non-target organisms, glyphosate-contaminated soils needed to be remediated, and bioremediation is a conventional remedial method. The success of this depends on the isolation of bacteria with the ability to degrade glyphosate. The goal of this study was to isolate glyphosate-degrading bacteria from the rhizosphere of maize and wheat with a repeated application history of glyphosate for 5–10 years and test their roles in promoting the growth of maize ( Zea mays ) and glyphosate degradation in vitro. Eleven isolated bacteria were inoculated, and their role in plant growth was compared at different levels (100 and 200 mg/kg) of glyphosate. The results revealed that E. ludwigii improved the highest shoot length by 26% and the root length by 34% compared to the control at 100 mg/kg. The relative water contents in leaves significantly improved by 58% using P. aeruginosa at 100 mg/kg. The maximum electrolyte leakage from leaves significantly reduced by 73% using E. ludwigii at 100 mg/kg compared to the control (uninoculated). A high-pressure liquid chromatography instrument was used to assess the glyphosate concentrations. The highest degradation of glyphosate was observed in treatments inoculated with E. ludwigii (99 and 40%), P. aeruginosa (95 and 39%), K. variicola , (91 and 38%) E. cloacae (92 and 38%), and S. liquefaciens (87 and 36%), respectively, at 100 and 200 mg/kg within 28 days. These five strains demonstrated a great potential for degrading glyphosate and promoting the growth of maize in vitro, and they will be further exploited for the biodegradation of glyphosate and the growth promotion of broader crop species in situ in the near future.

Suggested Citation

  • Waqas Mohy-Ud-Din & Muhammad Javed Akhtar & Safdar Bashir & Hafiz Naeem Asghar & Muhammad Farrakh Nawaz & Feng Chen, 2023. "Isolation of Glyphosate-Resistant Bacterial Strains to Improve the Growth of Maize and Degrade Glyphosate under Axenic Condition," Agriculture, MDPI, vol. 13(4), pages 1-19, April.
    • Handle: RePEc:gam:jagris:v:13:y:2023:i:4:p:886-:d:1125730
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/2077-0472/13/4/886/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/2077-0472/13/4/886/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Mohammad Shahid & Mohammad Tarique Zeyad & Asad Syed & Udai B. Singh & Abdullah Mohamed & Ali H. Bahkali & Abdallah M. Elgorban & John Pichtel, 2022. "Stress-Tolerant Endophytic Isolate Priestia aryabhattai BPR-9 Modulates Physio-Biochemical Mechanisms in Wheat ( Triticum aestivum L.) for Enhanced Salt Tolerance," IJERPH, MDPI, vol. 19(17), pages 1-26, September.
    2. Blessing Chidinma Nwachukwu & Ayansina Segun Ayangbenro & Olubukola Oluranti Babalola, 2021. "Elucidating the Rhizosphere Associated Bacteria for Environmental Sustainability," Agriculture, MDPI, vol. 11(1), pages 1-18, January.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.

      Corrections

      All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jagris:v:13:y:2023:i:4:p:886-:d:1125730. See general information about how to correct material in RePEc.

      If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

      If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

      If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

      For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

      Please note that corrections may take a couple of weeks to filter through the various RePEc services.

      IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.