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Transcriptome Analysis of Propylaea quatuordecimpunctata L. (Coleoptera: Coccinellidae) under High Temperature Stress

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  • Qing Yang

    (College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225007, China
    State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China)

  • Jinping Liu

    (State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China)

  • Yizhong Yang

    (College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225007, China)

  • Yanhui Lu

    (State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
    Western Agricultural Research Center, Chinese Academy of Agricultural Sciences, Changji 831100, China)

Abstract

In cotton-growing regions of northern Xinjiang, Propylaea quatuordecimpunctata (L.) (Coleoptera: Coccinellidae) is an important natural enemy that provides significant control of some pest hemipterans. Previous studies have shown that the survival and reproduction of P. quatuordecimpunctata differs under different high temperatures. However, its molecular mechanism for thermotolerance is poorly understood. In this study, transcriptome sequencing analysis was performed on P. quatuordecimpunctata , after its exposure to different temperatures (32–38 °C) for 24 h, using high-throughput sequencing technology. The results showed that the 35 vs. 38 °C groups had the most DEGs (1425), indicating that P. quatuordecimpunctata has more genes that can be mobilized under high temperature (38 °C). The results of functional analysis showed that the DEGs were mainly involved in “Oxidation–reduction process”, “Oxidoreductase activity”, “Metabolic pathways”, and “Small molecule metabolic processing” groups. We randomly selected DEGs (eleven P450 genes and one Hsp70 gene) of interest for qRT-PCR validation. The qRT-PCR results were consistent with the transcriptome data, indicating that the transcriptome data were reliable. In summary, these genes involved in these pathways play an important role in the resistance of P. quatuordecimpunctata to high temperature stress. Our study enriched our understanding of the molecular mechanism for thermotolerance in P. quatuordecimpunctata .

Suggested Citation

  • Qing Yang & Jinping Liu & Yizhong Yang & Yanhui Lu, 2022. "Transcriptome Analysis of Propylaea quatuordecimpunctata L. (Coleoptera: Coccinellidae) under High Temperature Stress," Agriculture, MDPI, vol. 12(8), pages 1-16, July.
    • Handle: RePEc:gam:jagris:v:12:y:2022:i:8:p:1088-:d:870178
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    References listed on IDEAS

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    1. Yanhui Lu & Kongming Wu & Yuying Jiang & Yuyuan Guo & Nicolas Desneux, 2012. "Widespread adoption of Bt cotton and insecticide decrease promotes biocontrol services," Nature, Nature, vol. 487(7407), pages 362-365, July.
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    Cited by:

    1. Xin-Yue Tian & Yu Gao & Muhammad Yasir Ali & Xiao-Hua Li & Ying-Lu Hu & Wen-Bo Li & Zi-Jie Wang & Shu-Sen Shi & Jin-Ping Zhang, 2022. "Impact of Temperature on Age–Stage, Two-Sex Life Table Analysis of a Chinese Population of Bean Bug, Riptortus pedestris (Hemiptera: Alydidae)," Agriculture, MDPI, vol. 12(9), pages 1-14, September.

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