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A Facile and Modified Scheme for Synchronization and Isolation of Nematode Eggs

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  • Satish Kumar Rajasekharan

    (School of Chemical Engineering, Yeungnam University, Gyeongsan 38541, Korea
    Department of Food Sciences, Institute of Postharvest Technology and Food Sciences, Agricultural Research Organization (ARO), The Volcani Center, Rishon LeZion 7528809, Israel
    These authors contributed equally to this work.)

  • Chaitany Jayprakash Raorane

    (School of Chemical Engineering, Yeungnam University, Gyeongsan 38541, Korea
    These authors contributed equally to this work.)

  • Jintae Lee

    (School of Chemical Engineering, Yeungnam University, Gyeongsan 38541, Korea)

Abstract

Nematodes are common pests that damage agricultural crop plants. Some of them are beneficial while others are parasitic and harmful to plants, animals and humans. Several in vitro studies have aimed to develop chemicals to kill parasitic nematodes, while others have been conducted to use beneficial nematodes as biocontrol agents. However, the preparation of large quantities of nematode eggs in a laboratory setting is challenging. Traditional egg isolation protocols involve the use of sieves to filter eggs or the use of chemicals that can be harmful to nematodes while isolating the eggs. Our method utilizes 1.5 × 1.5 cm sized chunks of bacterial or fungal feed to lure nematodes. A subsequent gentle washing of the consumed chunk with distilled water provides a rapid and straightforward method of collecting eggs in 6-well polystyrene plates and removing unwanted nematodes. Approximately 4000 Bursaphelenchus xylophilus eggs from a fungal chunk and 2400 Caenorhabditis elegans eggs from a bacterial chunk were obtained when tested. This study shows a protocol for the isolation of eggs and synchronization of nematode stages that is relatively straightforward, rapid, eco-friendly, and efficient. The protocol also provides a chemical-free and a reliable, simple means of separating eggs from adults and induces the synchronization of nematodes based on the simple concept that gravid nematodes can be provoked to lay eggs by providing additional feed.

Suggested Citation

  • Satish Kumar Rajasekharan & Chaitany Jayprakash Raorane & Jintae Lee, 2021. "A Facile and Modified Scheme for Synchronization and Isolation of Nematode Eggs," Agriculture, MDPI, vol. 11(7), pages 1-7, July.
  • Handle: RePEc:gam:jagris:v:11:y:2021:i:7:p:676-:d:596431
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    References listed on IDEAS

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    1. Joseph Nsengimana & Lander Bauters & Annelies Haegeman & Godelieve Gheysen, 2013. "Silencing of Mg - pat-10 and Mg - unc-87 in the Plant Parasitic Nematode Meloidogyne graminicola Using siRNAs," Agriculture, MDPI, vol. 3(3), pages 1-12, September.
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