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Feasibility of triacylglycerol production for biodiesel, utilizing Rhodococcus opacus as a biocatalyst and fishery waste as feedstock

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  • Palmer, Jacob D.
  • Brigham, Christopher J.

Abstract

Triacylglycerols (TAGs) can be produced via bacterial fermentation by the oleaginous Gram-positive microorganism Rhodococcus opacus strain PD630 in regulated, nutrient-deprived conditions with sufficient carbon supply. Microbially produced TAGs may be further refined via transesterification into biodiesel and glycerol, with 3mole of biodiesel and 1mole of glycerol produced from every 1mole of TAG by chemical conversion. Large-scale industrial production of biodiesel has been conducted for over a decade, yet microbially derived biodiesel has been, up to this point, absent from the biodiesel market. The use of a novel feedstock, chitin, from New England fishery waste may present a viable, cost-effective, unexplored carbon feedstock source for local biodiesel development. Availability and implementation of chitin as a feedstock, along with analysis of potential fuel characteristics, yield promising results for future industrial development of biodiesel production from R. opacus PD630 TAGs in regional locations with large lobster, shrimp, and crab harvesting operations around the world. With declining resources of fossil fuels and increased societal awareness of carbon emissions and climate change, an analytical review of this nature is critically relevant.

Suggested Citation

  • Palmer, Jacob D. & Brigham, Christopher J., 2016. "Feasibility of triacylglycerol production for biodiesel, utilizing Rhodococcus opacus as a biocatalyst and fishery waste as feedstock," Renewable and Sustainable Energy Reviews, Elsevier, vol. 56(C), pages 922-928.
    • Handle: RePEc:eee:rensus:v:56:y:2016:i:c:p:922-928
    DOI: 10.1016/j.rser.2015.12.002
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    1. Yong Jun Choi & Sang Yup Lee, 2013. "Microbial production of short-chain alkanes," Nature, Nature, vol. 502(7472), pages 571-574, October.
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    4. Nevena Lukovic & Dejan Bezbradica & Zorica Knezevic-Jugovic, 2011. "Biodiesel Fuel Production by Enzymatic Transesterification of Oils: Recent Trends, Challenges and Future Perspectives," Chapters, in: Maximino Manzanera (ed.), Alternative Fuel, IntechOpen.
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