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Production of ethanol from cassava pulp via fermentation with a surface-engineered yeast strain displaying glucoamylase

Author

Listed:
  • Kosugi, Akihiko
  • Kondo, Akihiko
  • Ueda, Mitsuyoshi
  • Murata, Yoshinori
  • Vaithanomsat, Pilanee
  • Thanapase, Warunee
  • Arai, Takamitsu
  • Mori, Yutaka

Abstract

Cassava (Manihot esculenta Crantz) pulp, produced in large amounts as a by-product of starch manufacturing, is a major biomass resource in Southeast Asian countries. It contains abundant starch (approximately 60%) and cellulose fiber (approximately 20%). To effectively utilize the cassava pulp, an attempt was made to convert its components to ethanol using a sake-brewing yeast displaying glucoamylase on the cell surface. Saccharomyces cerevisiae Kyokai no. 7 (strain K7) displaying Rhizopus oryzae glucoamylase, designated strain K7G, was constructed using the C-terminal-half region of α-agglutinin. A sample of cassava pulp was pretreated with a hydrothermal reaction (140°C for 1h), followed by treatment with a Trichoderma reesei cellulase to hydrolyze the cellulose in the sample. The K7G strain fermented starch and glucose in pretreated samples without addition of amylolytic enzymes, and produced ethanol in 91% and 80% of theoretical yield from 5% and 10% cassava pulp, respectively.

Suggested Citation

  • Kosugi, Akihiko & Kondo, Akihiko & Ueda, Mitsuyoshi & Murata, Yoshinori & Vaithanomsat, Pilanee & Thanapase, Warunee & Arai, Takamitsu & Mori, Yutaka, 2009. "Production of ethanol from cassava pulp via fermentation with a surface-engineered yeast strain displaying glucoamylase," Renewable Energy, Elsevier, vol. 34(5), pages 1354-1358.
    • Handle: RePEc:eee:renene:v:34:y:2009:i:5:p:1354-1358
    DOI: 10.1016/j.renene.2008.09.002
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    Cited by:

    1. Chatchai Kaewpila & Suwit Thip-uten & Anusorn Cherdthong & Waroon Khota, 2021. "Impact of Cellulase and Lactic Acid Bacteria Inoculant to Modify Ensiling Characteristics and In Vitro Digestibility of Sweet Corn Stover and Cassava Pulp Silage," Agriculture, MDPI, vol. 11(1), pages 1-12, January.
    2. Adekunle, Ademola & Orsat, Valerie & Raghavan, Vijaya, 2016. "Lignocellulosic bioethanol: A review and design conceptualization study of production from cassava peels," Renewable and Sustainable Energy Reviews, Elsevier, vol. 64(C), pages 518-530.
    3. Thangavelu, Saravana Kannan & Ahmed, Abu Saleh & Ani, Farid Nasir, 2016. "Review on bioethanol as alternative fuel for spark ignition engines," Renewable and Sustainable Energy Reviews, Elsevier, vol. 56(C), pages 820-835.
    4. Li, Lin & Sun, Zeyi & Yao, Xufeng & Wang, Donghai, 2016. "Optimal production scheduling for energy efficiency improvement in biofuel feedstock preprocessing considering work-in-process particle separation," Energy, Elsevier, vol. 96(C), pages 474-481.
    5. Yang, Peizhou & Guo, Liqiong & Cheng, Shujie & Lou, Nannan & Lin, Junfang, 2011. "Recombinant multi-functional cellulase activity in submerged fermentation of lignocellulosic wastes," Renewable Energy, Elsevier, vol. 36(12), pages 3268-3272.
    6. Rui Pacheco & Carla Silva, 2019. "Global Warming Potential of Biomass-to-Ethanol: Review and Sensitivity Analysis through a Case Study," Energies, MDPI, vol. 12(13), pages 1-18, July.
    7. Eckert, C.T. & Frigo, E.P. & Albrecht, L.P. & Albrecht, A.J.P. & Christ, D. & Santos, W.G. & Berkembrock, E. & Egewarth, V.A., 2018. "Maize ethanol production in Brazil: Characteristics and perspectives," Renewable and Sustainable Energy Reviews, Elsevier, vol. 82(P3), pages 3907-3912.
    8. Thangavelu, Saravana Kannan & Ahmed, Abu Saleh & Ani, Farid Nasir, 2014. "Bioethanol production from sago pith waste using microwave hydrothermal hydrolysis accelerated by carbon dioxide," Applied Energy, Elsevier, vol. 128(C), pages 277-283.
    9. Naqvi, Salman Raza & Jamshaid, Sana & Naqvi, Muhammad & Farooq, Wasif & Niazi, Muhammad Bilal Khan & Aman, Zaeem & Zubair, Muhammad & Ali, Majid & Shahbaz, Muhammad & Inayat, Abrar & Afzal, Waheed, 2018. "Potential of biomass for bioenergy in Pakistan based on present case and future perspectives," Renewable and Sustainable Energy Reviews, Elsevier, vol. 81(P1), pages 1247-1258.

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