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Spatially-ordered layer-by-layer biofilms of a two-species microbial consortium promote hydrogen production

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  • Li, Jiangbo
  • Wang, Kai
  • Wang, Shaojie
  • Su, Haijia

Abstract

The low hydrogen production efficiency caused by volatile fatty acids stress is the main technical barrier in the field of biohydrogen production. To enhance the resistance to acid stress and improve the synergistic efficiency of hydrogen-producing bacteria, spatially-ordered layer-by-layer biofilms of a two-species microbial consortium, consisting of Bacillus cereus A1 and Brevumdimonas naejangsanensis B1, were developed. The results showed that the spatially-ordered biofilms (SBs) significantly improved the cooperation efficiency of the two strains, and the hydrogen production increased by 103.27% and 66.50% compared with suspended fermentation and mixed biofilms (MBs) fermentation, respectively. Furthermore, the mixed ratios of the two strains were further optimized, and the highest hydrogen accumulation of 1755 mL L−1 was obtained at the inoculation volume ratio of A1:B1 = 1:1. Finally, the biofilms were used for 5 repeat batches of fermentation, and the hydrogen production performance was stable, with an average hydrogen accumulation of 1736 mL L−1. This study proposed a layer-by-layer biofilm hydrogen production system based on the spatially ordered distribution of functional bacteria, which significantly improved the cooperation efficiency and stability of the two-species microbial consortium.

Suggested Citation

  • Li, Jiangbo & Wang, Kai & Wang, Shaojie & Su, Haijia, 2023. "Spatially-ordered layer-by-layer biofilms of a two-species microbial consortium promote hydrogen production," Renewable Energy, Elsevier, vol. 215(C).
    • Handle: RePEc:eee:renene:v:215:y:2023:i:c:s0960148123008029
    DOI: 10.1016/j.renene.2023.118905
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    References listed on IDEAS

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    1. Jie Mei & Huize Chen & Qiang Liao & Abdul-Sattar Nizami & Ao Xia & Yun Huang & Xianqing Zhu & Xun Zhu, 2020. "Effects of Operational Parameters on Biofilm Formation of Mixed Bacteria for Hydrogen Fermentation," Sustainability, MDPI, vol. 12(21), pages 1-15, October.
    2. Wang, Shaojie & Ma, Zhihong & Su, Haijia, 2018. "Two-step continuous hydrogen production by immobilized mixed culture on corn stalk," Renewable Energy, Elsevier, vol. 121(C), pages 230-235.
    3. Mockaitis, Gustavo & Bruant, Guillaume & Guiot, Serge R. & Peixoto, Guilherme & Foresti, Eugenio & Zaiat, Marcelo, 2020. "Acidic and thermal pre-treatments for anaerobic digestion inoculum to improve hydrogen and volatile fatty acid production using xylose as the substrate," Renewable Energy, Elsevier, vol. 145(C), pages 1388-1398.
    4. Ma, Zhihong & Li, Chan & Su, Haijia, 2017. "Dark bio-hydrogen fermentation by an immobilized mixed culture of Bacillus cereus and Brevumdimonas naejangsanensis," Renewable Energy, Elsevier, vol. 105(C), pages 458-464.
    5. Wen, Han-Quan & Xing, De-Feng & Xie, Guo-Jun & Yin, Tian-Ming & Ren, Nan-Qi & Liu, Bing-Feng, 2019. "Enhanced photo-fermentative hydrogen production by synergistic effects of formed biofilm and added L-cysteine," Renewable Energy, Elsevier, vol. 139(C), pages 643-650.
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