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Flow cytometry assay to evaluate lipid production by the marine microalga Tetraselmis sp. using a two stage process

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  • Dammak, Mouna
  • Ben Hlima, Hajer
  • Elleuch, Fatma
  • Pichon, Chantal
  • Michaud, Philippe
  • Fendri, Imen
  • Abdelkafi, Slim

Abstract

Tetraselmis sp. strain MD-01, a microalga isolated from the Tunisian marine coast, has been studied for autotrophic lipids production with two stage process. This strategy was conducted for maximum cells production under moderated conditions (2.12 g L−1), followed by cultivation under stressed conditions to induce lipid synthesis. High irradiance (300 μmol m−2 s−1) and nitrogen depletion (S mode) showed better carotenoids accumulation and lower chlorophylls content in microalgae compared to those obtained in biomass cultivated under high irradiance (Sg mode). This two-stage strategy allowed to increase of lipid content in cells cultivated in Sg and S modes from 18% DW (stage I) to 50 and 61.5% DW (stage II), respectively, with an increase of saturated and long chain fatty acids levels. Based on fluorometry analyses, an increase of lipids in Nile-Red stained cells and a decrease of chlorophylls auto-fluorescence under stressed conditions were shown. From spectral data, the lipids: amide I ratio obtained from S mode was 2.17 and 4.45 times higher than that from Sg mode and control conditions, respectively. These results revealed the potential of Tetraselmis strain MD-01 for biodiesel production.

Suggested Citation

  • Dammak, Mouna & Ben Hlima, Hajer & Elleuch, Fatma & Pichon, Chantal & Michaud, Philippe & Fendri, Imen & Abdelkafi, Slim, 2021. "Flow cytometry assay to evaluate lipid production by the marine microalga Tetraselmis sp. using a two stage process," Renewable Energy, Elsevier, vol. 177(C), pages 280-289.
    • Handle: RePEc:eee:renene:v:177:y:2021:i:c:p:280-289
    DOI: 10.1016/j.renene.2021.05.126
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    References listed on IDEAS

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    1. Ra, Chae Hun & Kang, Chang-Han & Kim, Na Kyoung & Lee, Choul-Gyun & Kim, Sung-Koo, 2015. "Cultivation of four microalgae for biomass and oil production using a two-stage culture strategy with salt stress," Renewable Energy, Elsevier, vol. 80(C), pages 117-122.
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    Cited by:

    1. Zhao, Yongteng & Qiao, Tengsheng & Gu, Dan & Zhu, Liyan & Yu, Xuya, 2022. "Stimulating biolipid production from the novel alga Ankistrodesmus sp. by coupling salt stress and chemical induction," Renewable Energy, Elsevier, vol. 183(C), pages 480-490.
    2. Monteiro, Ivo & Schüler, Lisa M. & Santos, Eunice & Pereira, Hugo & Schulze, Peter S.C. & Florindo, Cláudia & Varela, João & Barreira, Luísa, 2023. "Two-stage lipid induction in the microalga Tetraselmis striata CTP4 upon exposure to different abiotic stresses," Renewable Energy, Elsevier, vol. 208(C), pages 693-701.

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