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Co-cultivation of two engineered strains of Synechocystis sp. PCC 6803 results in improved bioethanol production

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  • Velmurugan, Rajendran
  • Incharoensakdi, Aran

Abstract

The Synechocystis sp. PCC 6803 is a promising host for ethanol biosynthesis from CO2. In this study, the production of ethanol was performed by utilizing two different engineered Synechocystis strains consisting of pdc-adh (pyruvate decarboxylase and alcohol dehydrogenase) overexpression (▲APX) and glgC-phaA (glucose-1-phosphate adenylyltransferase and PHA-specific β-ketothiolase) knockout (ΔGBK) pathways. This strategy involves destruction of glycogen and polyhydroxybutyrate (PHB) synthesis, both of which act as a storage polymer. The blocking of such biosynthetic pathways enabled Synechocystis to release various metabolites into the medium with an increase of chlorophyll a content and oxygen evolution due to the reduction of glycogen and PHB synthesis. In co-cultivation system, the extracellular metabolites released from knock out strain (ΔGBK) were utilized by the pdc-adh overexpressing strain (▲APX) and produced 4708 mg/L ethanol, which is comparatively higher than pdc-adh overexpressing strain containing glgC and PhaA knock out (ΔGBK-▲APX) (4103 mg/L). Although the concentration of ▲APX cells were lower in co-culture than in mono-culture, the overflow of metabolites from knock out Synechocystis and the consumption of those products by pdc-adh overexpressing Synechocystis in co-cultivation system improved the overall yield.

Suggested Citation

  • Velmurugan, Rajendran & Incharoensakdi, Aran, 2020. "Co-cultivation of two engineered strains of Synechocystis sp. PCC 6803 results in improved bioethanol production," Renewable Energy, Elsevier, vol. 146(C), pages 1124-1133.
    • Handle: RePEc:eee:renene:v:146:y:2020:i:c:p:1124-1133
    DOI: 10.1016/j.renene.2019.07.025
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    1. Naveena, Balakrishnan & Armshaw, Patricia & Pembroke, J. Tony & Gopinath, Kannapan Panchamoorthy, 2016. "Kinetic and optimisation studies on ultrasonic intensified photo-autotrophic ethanol production from Synechocystis sp," Renewable Energy, Elsevier, vol. 95(C), pages 522-530.
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    Cited by:

    1. Varsha K. Singh & Sapana Jha & Palak Rana & Renu Soni & Rowland Lalnunpuii & Prashant K. Singh & Rajeshwar P. Sinha & Garvita Singh, 2024. "Cyanobacteria as a Biocatalyst for Sustainable Production of Biofuels and Chemicals," Energies, MDPI, vol. 17(2), pages 1-25, January.

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