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A mild extraction and separation procedure of polysaccharide, lipid, chlorophyll and protein from Chlorella spp

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  • Zhao, Wenhuan
  • Duan, Meirong
  • Zhang, Xu
  • Tan, Tianwei

Abstract

This study developed a way to separate lipid, polysaccharide, protein and chlorophyll from wet Chlorella spp. (85% moisture content, wt) by using three-phase partitioning (TPP). The optimum conditions for the key steps were researched and defined as (I) cell disruption at the following conditions: biomass concentration 20 g/L, bath temperature 90 °C, duration 2.5 h, enzyme concentration 12 g/L, enzyme hydrolysis time 4 h; and (II) TPP at 40% (NH4)2SO4 (wt, %), sample solution/t-butanol 1: 2 (V/V), room temperature. The extraction yield of lipid and the poysaccharide preservation rate by the optimized procedure were 80.24%, 90.63%, respectively. The protein removal rate was 78.87%. The results provided a promising towards the industrial separation of lipid, polysaccaride, protein and chlorophyll.

Suggested Citation

  • Zhao, Wenhuan & Duan, Meirong & Zhang, Xu & Tan, Tianwei, 2018. "A mild extraction and separation procedure of polysaccharide, lipid, chlorophyll and protein from Chlorella spp," Renewable Energy, Elsevier, vol. 118(C), pages 701-708.
    • Handle: RePEc:eee:renene:v:118:y:2018:i:c:p:701-708
    DOI: 10.1016/j.renene.2017.11.046
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    References listed on IDEAS

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